Day: November 23, 2022

Effective whole cell biotransformation of arginine to a four-carbon diamine putrescine using engineered Escherichia coli was written by Yang, Shih-Chen;Ting, Wan-Wen;Ng, I-Son. And the article was included in Biochemical Engineering Journal in 2022.Recommanded Product: (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate The following contents are mentioned in the article:

The rising awareness of environmental protection has triggered bio-based materials to replace the traditional petrochem. plastics. Putrescine as 1,4-diaminobutane is an important monomer of polyamide (PA) and uses in the sustainable chem. industry. Herein, a time-effective whole cell bioconversion of L-arginine to putrescine was developed, which has applied the key enzymes (i.e., SpeA and SpeB) from the arginine decarboxylase (ADC) pathway. The synergetic collaboration of both enzymes was examined from the different combination of plasmids among 4 Escherichia coli chassis. The optimal reaction condition was at pH 9 with 1 mM pyridoxal-5�phosphate (PLP) and 10 mM magnesium, thus 90% conversion was obtained using an all-in-one plasmid with equal protein of SpeA and SpeB in BL21(DE3). The enzymic kinetics demonstrated the higher k_cat of SpeA (1212 s^-1) than that of SpeB (418 s^-1), while severe inhibition of putrescine on SpeA (KI = 8.61 mM), thus it was disadvantage using the surface display of enzyme. To prevent the feedback-inhibition by product, a 2-step enzymic reaction with cold treatment was conducted. Finally, the putrescine was achieved 17.1 g/L with the productivity of 8.56 g/L/h under 86% conversion of 50 g/L -arginine-HCl, which is an effective approach to obtain high putrescine titer. This study involved multiple reactions and reactants, such as (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7Recommanded Product: (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate).

(4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7) belongs to pyridine derivatives. Pyridines are an important class of heterocycles and occur in polysubstituted forms in many naturally occurring biologically active compounds, drug molecules and chiral ligands. Pyridine groups exist in countless molecules, and their applications include catalysis, drug design, molecular recognition, and natural product synthesis.Recommanded Product: (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate

Referemce:
Pyridine – Wikipedia,
Pyridine | C5H5N – PubChem

Analyses of pre-steady-state kinetics and isotope effects of the γ-elimination reaction catalyzed by Citrobacter freundii methionine γ-lyase was written by Kuznetsova, Aleksandra A.;Faleev, Nicolai G.;Morozova, Elena A.;Anufrieva, Natalya V.;Gogoleva, Olga I.;Tsvetikova, Marina A.;Fedorova, Olga S.;Demidkina, Tatyana V.;Kuznetsov, Nikita A.. And the article was included in Biochimie in 2022.Related Products of 54-47-7 The following contents are mentioned in the article:

Methionine γ-lyase (MGL) is a pyridoxal 5â€?phosphate-dependent enzyme catalyzing γ-elimination in L-methionine. Pyridoxal 5â€?phosphate-dependent enzymes have unique spectral properties that allow to monitor sequential formation and decomposition of various intermediates via the detection of absorbance changes. The kinetic mechanism of the γ-elimination reaction catalyzed by Citrobacter freundii MGL was elucidated here by fast stopped-flow kinetic anal. Single-wavelength detection of characteristic absorbance changes enabled us to compare transformations of intermediates in the course of the reaction with different substrates. The influence of various γ-substituents in the substrate on the formation of key intermediates was estimated Kinetic isotope effects of α- and β-protons were determined using deuterium-substituted L-methionine. Contributions of amino acid residues Tyr113 and Tyr58 located in the active site on the formation and decomposition of reaction intermediates were identified too. α-Aminocrotonate formation is the rate-limiting step of the enzymic γ-elimination reaction. Kinetic isotope effects strongly support concerted reaction mechanisms of transformation between an external aldimine and a ketimine intermediate as well as a ketimine intermediate and an unsaturated ketimine. This study involved multiple reactions and reactants, such as (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7Related Products of 54-47-7).

(4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7) belongs to pyridine derivatives. Pyridines are an important class of heterocycles and occur in polysubstituted forms in many naturally occurring biologically active compounds, drug molecules and chiral ligands. Halopyridines are particularly attractive synthetic building blocks in a variety of cross-coupling methods, including the Suzuki-Miyaura cross-coupling reaction.Related Products of 54-47-7

Referemce:
Pyridine – Wikipedia,
Pyridine | C5H5N – PubChem

Gene set enrichment analysis and ingenuity pathway analysis to identify biomarkers in Sheng-ji Hua-yu formula treated diabetic ulcers was written by Ru, Yi;Zhang, Ying;Xiang, Yan-wei;Luo, Ying;Luo, Yue;Jiang, Jing-si;Song, Jian-kun;Fei, Xiao-ya;Yang, Dan;Zhang, Zhan;Zhang, Hui-ping;Liu, Tai-yi;Yin, Shuang-yi;Li, Bin;Kuai, Le. And the article was included in Journal of Ethnopharmacology in 2022.Application of 54-47-7 The following contents are mentioned in the article:

Sheng-ji Hua-yu (SJHY) formula is a Chinese herbal prescription for diabetic ulcers (DUs) treatment, which can accelerate wound reconstruction and shorten the healing time. However, its mechanism role maintains unclear. To elucidate the mol. mechanisms of SJHY application on DUs. To begin with, transcriptome sequencing was adopted to identified differentially expression mRNAs among normal ulcers, DUs, and DUs + SJHY treatment in vivo. Liquid chromatog.-tandem mass spectrometry was applied for the quality control of SJHY formula. GO and KEGG enrichment anal. were used to identify the mechanisms underlying the therapeutic effect of SJHY formula, and then gene set enrichment anal. and ingenuity pathway anal. were conducted for functional anal. Further, qPCR detection was performed in vivo for validation. SJHY administration could regulate the glucose metabolic process, AMPK and HIF-1 pathway to accelerate healing processes of DUs. Besides, CRHR1, SHH, and GAL were identified as the critical targets, and SLC6A3, GRP, FGF23, and CYP27B1 were considered as the upstream genes of SJHY treatment. Combined with animal experiments, the prediction results were validated in DUs mice model. This study used modular pharmacol. anal. to identify the biomarkers of SJHY formula and provide the potential therapeutic targets for DUs treatment as well. This study involved multiple reactions and reactants, such as (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7Application of 54-47-7).

(4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7) belongs to pyridine derivatives. Pyridine is diamagnetic and has a diamagnetic susceptibility of âˆ?8.7 × 10âˆ? cm3·molâˆ?.The molecular electric dipole moment is 2.2 debyes. The standard enthalpy of formation is 100.2 kJ·molâˆ? in the liquid phase and 140.4 kJ·molâˆ? in the gas phase. Pyridine derivatives are also useful as small-molecule α-helix mimetics that inhibit protein-protein interactions, as well as functionally selective GABA ligands.Application of 54-47-7

Referemce:
Pyridine – Wikipedia,
Pyridine | C5H5N – PubChem

Gene set enrichment analysis and ingenuity pathway analysis to identify biomarkers in Sheng-ji Hua-yu formula treated diabetic ulcers was written by Ru, Yi;Zhang, Ying;Xiang, Yan-wei;Luo, Ying;Luo, Yue;Jiang, Jing-si;Song, Jian-kun;Fei, Xiao-ya;Yang, Dan;Zhang, Zhan;Zhang, Hui-ping;Liu, Tai-yi;Yin, Shuang-yi;Li, Bin;Kuai, Le. And the article was included in Journal of Ethnopharmacology in 2022.Safety of (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate The following contents are mentioned in the article:

Sheng-ji Hua-yu (SJHY) formula is a Chinese herbal prescription for diabetic ulcers (DUs) treatment, which can accelerate wound reconstruction and shorten the healing time. However, its mechanism role maintains unclear. To elucidate the mol. mechanisms of SJHY application on DUs. To begin with, transcriptome sequencing was adopted to identified differentially expression mRNAs among normal ulcers, DUs, and DUs + SJHY treatment in vivo. Liquid chromatog.-tandem mass spectrometry was applied for the quality control of SJHY formula. GO and KEGG enrichment anal. were used to identify the mechanisms underlying the therapeutic effect of SJHY formula, and then gene set enrichment anal. and ingenuity pathway anal. were conducted for functional anal. Further, qPCR detection was performed in vivo for validation. SJHY administration could regulate the glucose metabolic process, AMPK and HIF-1 pathway to accelerate healing processes of DUs. Besides, CRHR1, SHH, and GAL were identified as the critical targets, and SLC6A3, GRP, FGF23, and CYP27B1 were considered as the upstream genes of SJHY treatment. Combined with animal experiments, the prediction results were validated in DUs mice model. This study used modular pharmacol. anal. to identify the biomarkers of SJHY formula and provide the potential therapeutic targets for DUs treatment as well. This study involved multiple reactions and reactants, such as (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7Safety of (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate).

(4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7) belongs to pyridine derivatives. Pyridine is diamagnetic and has a diamagnetic susceptibility of âˆ?8.7 × 10âˆ? cm3·molâˆ?.The molecular electric dipole moment is 2.2 debyes. The standard enthalpy of formation is 100.2 kJ·molâˆ? in the liquid phase and 140.4 kJ·molâˆ? in the gas phase. Many analogues of pyridine are known where N is replaced by other heteroatoms . Substitution of one C–H in pyridine with a second N gives rise to the diazine heterocycles (C4H4N2), with the names pyridazine, pyrimidine, and pyrazine.Safety of (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate

Referemce:
Pyridine – Wikipedia,
Pyridine | C5H5N – PubChem

Improving the activity and synergistic catalysis of L-aspartate β-decarboxylase by arginine introduction on the surface was written by Hao, Mingzhu;Cui, Ruizhi;Zhu, Xiaoqing;Han, Laichuang;Zhou, Zhemin;Liu, Zhongmei. And the article was included in Catalysis Science & Technology in 2022.HPLC of Formula: 54-47-7 The following contents are mentioned in the article:

Decarboxylase typically catalyzes reactions under acidic conditions, which is the drawback for its synergistic catalysis with other enzymes because most enzymes catalyze reactions under near neutral conditions. Improving the activity of decarboxylase under near neutral conditions will greatly contribute to its application in synthetic biol. and multienzyme cascade catalysis for the production of chem. compounds, in which many enzymes need to function cooperatively. We choose L-aspartate β-decarboxylase (ASD) that is widely applied in industry to implement the semirational design strategy. The catalytic ability of ASD was successfully improved by substituting glutamic acid for arginine at the 88th position on the surface. The specific activity of an E88R variant was increased to 481 U mg-1 compared to 230 U mg-1 for the wild type. Furthermore, the stability over a range of pH 3.0-8.0 was dramatically increased. The catalytic ability and synergy with other enzymes of the E88R variant were demonstrated in a triple-enzyme system for L-alanine production, and the productivity was increased from 28.2 g (L-1 h-1) to 43.3 g (L-1 h-1). The pH-dependent inactivation of ASD was relieved, and this strategy could be expanded to the decarboxylase family. Addnl., the variant obtained here also shows promising prospects for application in industry. This study involved multiple reactions and reactants, such as (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7HPLC of Formula: 54-47-7).

(4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7) belongs to pyridine derivatives. Pyridines are an important class of heterocycles and occur in polysubstituted forms in many naturally occurring biologically active compounds, drug molecules and chiral ligands. Halopyridines are particularly attractive synthetic building blocks in a variety of cross-coupling methods, including the Suzuki-Miyaura cross-coupling reaction.HPLC of Formula: 54-47-7

Referemce:
Pyridine – Wikipedia,
Pyridine | C5H5N – PubChem

Transcriptome analysis of the effect of high-temperature on nutrient metabolism in juvenile grass carp (Ctenopharyngodon idellus) was written by Huang, Dongyu;Ren, Mingchun;Liang, Hualiang;Ge, Xianping;Xu, Hao;Wu, Longhua. And the article was included in Gene in 2022.Category: pyridine-derivatives The following contents are mentioned in the article:

To investigate the variations in gene expression in grass carp under high-temperature stress, two libraries were constructed from a high-temperature treatment group (T33) and a control group (T27) and sequenced using Illumina sequencing technol. The results showed that sequencing generated a total of 279,398,348 raw reads, approx. 40.7-51.8 M clean reads were obtained from each library, and the percentage of uniquely mapped transcripts ranged from 80.13 to 84.58%. A total of 260 differentially expressed genes (DEGs) were identified under high-temperature stress, among which 84 genes were upregulated and 176 genes were downregulated. Ten DEGs were randomly selected for quant. RT-PCR (qRT-PCR) anal., and the results confirmed that the transcriptome anal. was reliable. Furthermore, the DEGs were subjected to Gene Ontol. (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment anal., and the results showed that most of the DEGs were involved in protein, lipid and carbohydrate metabolism Moreover, plasma urea nitrogen (Urea) and triglyceride (TG) contents were significantly lower in the high-temperature treatment group than in the control group (P < 0.01). In summary, these results indicated that high-temperature stress could inhibit protein synthesis, decrease fatty acid synthesis, and weaken carbohydrate metabolism in juvenile grass carp. This study involved multiple reactions and reactants, such as (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7Category: pyridine-derivatives).

(4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7) belongs to pyridine derivatives. Pyridine has a dipole moment and a weaker resonant stabilization than benzene (resonance energy 117 kJ·mol� in pyridine vs. 150 kJ·mol� in benzene). Several pyridine derivatives play important roles in biological systems. While its biosynthesis is not fully understood, nicotinic acid (vitamin B3) occurs in some bacteria, fungi, and mammals.Category: pyridine-derivatives

Referemce:
Pyridine – Wikipedia,
Pyridine | C5H5N – PubChem

Transcriptome analysis of the effect of high-temperature on nutrient metabolism in juvenile grass carp (Ctenopharyngodon idellus) was written by Huang, Dongyu;Ren, Mingchun;Liang, Hualiang;Ge, Xianping;Xu, Hao;Wu, Longhua. And the article was included in Gene in 2022.HPLC of Formula: 54-47-7 The following contents are mentioned in the article:

To investigate the variations in gene expression in grass carp under high-temperature stress, two libraries were constructed from a high-temperature treatment group (T33) and a control group (T27) and sequenced using Illumina sequencing technol. The results showed that sequencing generated a total of 279,398,348 raw reads, approx. 40.7-51.8 M clean reads were obtained from each library, and the percentage of uniquely mapped transcripts ranged from 80.13 to 84.58%. A total of 260 differentially expressed genes (DEGs) were identified under high-temperature stress, among which 84 genes were upregulated and 176 genes were downregulated. Ten DEGs were randomly selected for quant. RT-PCR (qRT-PCR) anal., and the results confirmed that the transcriptome anal. was reliable. Furthermore, the DEGs were subjected to Gene Ontol. (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment anal., and the results showed that most of the DEGs were involved in protein, lipid and carbohydrate metabolism Moreover, plasma urea nitrogen (Urea) and triglyceride (TG) contents were significantly lower in the high-temperature treatment group than in the control group (P < 0.01). In summary, these results indicated that high-temperature stress could inhibit protein synthesis, decrease fatty acid synthesis, and weaken carbohydrate metabolism in juvenile grass carp. This study involved multiple reactions and reactants, such as (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7HPLC of Formula: 54-47-7).

(4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7) belongs to pyridine derivatives. In contrast to benzene, Pyridine’s electron density is not evenly distributed over the ring, reflecting the negative inductive effect of the nitrogen atom. Pyridine derivatives are also useful as small-molecule α-helix mimetics that inhibit protein-protein interactions, as well as functionally selective GABA ligands.HPLC of Formula: 54-47-7

Referemce:
Pyridine – Wikipedia,
Pyridine | C5H5N – PubChem

Deep metagenomic characterization of gut microbial community and function in preeclampsia. was written by Lv, Li-Juan;Li, Sheng-Hui;Wen, Ji-Ying;Wang, Guang-Yang;Li, Hui;He, Tian-Wen;Lv, Qing-Bo;Xiao, Man-Chun;Duan, Hong-Li;Chen, Min-Chai;Yi, Zhou-Ting;Yan, Qiu-Long;Yin, Ai-Hua. And the article was included in Frontiers in cellular and infection microbiology in 2022.Application In Synthesis of (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate The following contents are mentioned in the article:

Preeclampsia (PE) is a pregnancy complication characterized by severe hypertension and multiple organ damage. Gut microbiota has been linked to PE by previous amplicon sequencing studies. To resolve the PE gut microbiota in a higher taxonomy resolution, we performed shotgun metagenomic sequencing on the fecal samples from 40 early-onset PE and 37 healthy pregnant women. We recovered 1,750 metagenome-assembled genomes (representing 406 species) from the metagenomic dataset and profiled their abundances. We found that PE gut microbiota had enriched in some species belonging to Blautia, Pauljensenia, Ruminococcus, and Collinsella and microbial functions such as the bacitracin/lantibiotics transport system, maltooligosaccharide transport system, multidrug efflux pump, and rhamnose transport system. Conversely, the gut microbiome of healthy pregnant women was enriched in species of Bacteroides and Phocaeicola and microbial functions including the porphyrin and chlorophyll metabolism, pyridoxal-P biosynthesis, riboflavin metabolism, and folate biosynthesis pathway. PE diagnostic potential of gut microbial biomarkers was developed using both species and function profile data. These results will help to explore the relationships between gut bacteria and PE and provide new insights into PE early warning. This study involved multiple reactions and reactants, such as (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7Application In Synthesis of (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate).

(4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7) belongs to pyridine derivatives. Pyridines are an important class of heterocycles and occur in polysubstituted forms in many naturally occurring biologically active compounds, drug molecules and chiral ligands. Several pyridine derivatives play important roles in biological systems. While its biosynthesis is not fully understood, nicotinic acid (vitamin B3) occurs in some bacteria, fungi, and mammals.Application In Synthesis of (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate

Referemce:
Pyridine – Wikipedia,
Pyridine | C5H5N – PubChem

The regulating effect of Tibet Opuntia ficus-indica (Linn.) Mill. polysaccharides on the intestinal flora of cyclophosphamide-induced immunocompromised mice was written by Liu, Zhendong;Zhang, Jinchao;Zhao, Qian;Wen, Aomei;Li, Liang;Zhang, Yu. And the article was included in International Journal of Biological Macromolecules in 2022.Application In Synthesis of (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate The following contents are mentioned in the article:

The stem of Opuntia species, a traditional medicinal plant, is widely used as food and functional raw material because of its rich polysaccharide content. There have been many studies on the immune function of polysaccharides from Opuntia stem, but only few have examined this function with respect to intestinal microbes. In this study, the effects of different concentrations of Opuntia stem polysaccharides on the immunity and intestinal microflora of cyclophosphamide (CTX)-induced immunocompromised mice were explored. The results showed that Tibet Opuntia ficus-indica (Linn.) Mill. polysaccharides (ODPs) could effectively increase the white blood cells (WBC) count index of mice and improve their thymus and spleen indexes, while effectively promoting the secretion of IL-4, IL-1β, TNF-α and IFN-γ, with these effects being dependent on the concentration of crude polysaccharides. The intake of ODPs significantly regulated the relative abundance of Lactobacillus, Bacteroides and Akkermansia, and the new dominant intestinal bacterial species were Deferribacteres, Actinomycetes, Firmicutes, Tenericutes, Actinomycetes and Pasteurella. In addition, the ODPs could effectively enhance the metabolic level of lysine synthesis and decomposition, regulate the gene expression level after immune disorders, and enhance the overall health of the immunodeficient mice. This study involved multiple reactions and reactants, such as (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7Application In Synthesis of (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate).

(4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7) belongs to pyridine derivatives. Pyridines are an important class of heterocycles and occur in polysubstituted forms in many naturally occurring biologically active compounds, drug molecules and chiral ligands. Several pyridine derivatives play important roles in biological systems. While its biosynthesis is not fully understood, nicotinic acid (vitamin B3) occurs in some bacteria, fungi, and mammals.Application In Synthesis of (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate

Referemce:
Pyridine – Wikipedia,
Pyridine | C5H5N – PubChem