Dan, Krishna; Veetil, Aneesh T.; Chakraborty, Kasturi; Krishnan, Yamuna published the artcile< DNA nanodevices map enzymatic activity in organelles>, Safety of 1,2-Di(pyridin-2-yl)disulfane, the main research area is DNA nanodevice endosomal fluorescent imaging.
Cellular reporters of enzyme activity are based on either fluorescent proteins or small mols. Such reporters provide information corresponding to wherever inside cells the enzyme is maximally active and preclude minor populations present in subcellular compartments. Here the authors describe a chem. imaging strategy to selectively interrogate minor, subcellular pools of enzymic activity. This new technol. confines the detection chem. to a designated organelle, enabling imaging of enzymic cleavage exclusively within the organelle. The authors have thus quant. mapped disulfide reduction exclusively in endosomes in Caenorhabditis elegans and identified that exchange is mediated by minor populations of the enzymes PDI-3 and TRX-1 resident in endosomes. Impeding intraendosomal disulfide reduction by knocking down TRX-1 protects nematodes from infection by Corynebacterium diphtheriae, revealing the importance of this minor pool of endosomal TRX-1. TRX-1 also mediates endosomal disulfide reduction in human cells. A range of enzymic cleavage reactions in organelles are amenable to anal. by this new reporter strategy.
Nature Nanotechnology published new progress about Caenorhabditis elegans. 2127-03-9 belongs to class pyridine-derivatives, and the molecular formula is C10H8N2S2, Safety of 1,2-Di(pyridin-2-yl)disulfane.