Garcia-Cuellar, Claudia Maria; Hernandez-Delgadillo, Rene; Solis-Soto, Juan Manuel; Meester, Irene; Sanchez-Perez, Yesennia; Nakagoshi-Cepeda, Sergio Eduardo; Nakagoshi-Cepeda, Maria Argelia Akemi; Chellam, Shankararaman; Cabral-Romero, Claudio published the artcile< Cetylpyridinium chloride inhibits human breast tumor cells growth in a no-selective way>, Synthetic Route of 123-03-5, the main research area is breast tumor cell growth cetylpyridinium chloride anticancer; Antitumor activity; LD50 assay; cetylpyridinium chloride; chemotherapy; human breast cancer; quaternary ammonium salts.
Objective: Analyze the antitumor capacity of cetylpyridinium chloride (CPC) on human breast tumor cells, and the possible action mechanism. Material and methods: The human breast tumor cells MCF-7 and no-tumor breast cells MCF-10A were exposed to CPC under various condition (concentration and duration). Cell viability was measured with MTT assay, the LIVE/DEAD assay, and fluorescence microscopy. Membrane permeability after CPC exposure was evaluated by Calcein AM assay, mitochondrial morphol. with a MitoView staining, and genotoxicity with the comet assay and fluorescence microscopy. Results: CPC was cytotoxic to both MCF-7 and MCF-10A as of a 24-h exposure to 0.1μM. Cytotoxicity was dosedependent and reached 91for MCF-7 and 78for MCF-10A after a 24-h exposure to 100μM CPC, which outperformed the pos. control doxorubicin in effectiveness and selectivity. The LD50 of CPC on was 6μM for MCF-7 and 8μM for MCF-10A, yielding a selectivity index of 1.41. With respect to the action mechanisms, the comet assay did not reveal genome fragmentation. On the other hand, membrane damage was dose-dependent and may also affect mitochondrial morphol. Conclusion: Cetylpyridinium chloride inhibits MCF-7 cell growing in a non-selective way as of 5min of exposure. The action mechanism of CPC on tumor cells involves cell membrane damage without change neither mitochondrial morphol. nor genotoxicity.
Journal of Applied Biomaterials & Functional Materials published new progress about Antitumor agents. 123-03-5 belongs to class pyridine-derivatives, and the molecular formula is C21H38ClN, Synthetic Route of 123-03-5.