Jiao, Chen; Obst, Franziska; Geisler, Martin; Che, Yunjiao; Richter, Andreas; Appelhans, Dietmar; Gaitzsch, Jens; Voit, Brigitte published the artcile< Reversible Protein Capture and Release by Redox-Responsive Hydrogel in Microfluidics>, Product Details of C10H8N2S2, the main research area is protein capture redox responsive hydrogel microfluidics; disulfide bonds; hydrogels; mechanical properties; microfluidics; protein capture and release; redox-responsive; swelling behaviors.
Stimuli-responsive hydrogels have a wide range of potential applications in microfluidics, which has drawn great attention. Double crosslinked hydrogels are very well suited for this application as they offer both stability and the required responsive behavior. Here, we report the integration of poly(N-isopropylacrylamide) (PNiPAAm) hydrogel with a permanent crosslinker (N,N′-methylenebisacrylamide, BIS) and a redox responsive reversible crosslinker (N,N′-bis(acryloyl)cystamine, BAC) into a microfluidic device through photopolymerization Cleavage and re-formation of disulfide bonds introduced by BAC changed the crosslinking densities of the hydrogel dots, making them swell or shrink. Rheol. measurements allowed for selecting hydrogels that withstand long-term shear forces present in microfluidic devices under continuous flow. Once implemented, the thiol-disulfide exchange allowed the hydrogel dots to successfully capture and release the protein bovine serum albumin (BSA). BSA was labeled with rhodamine B and functionalized with 2-(2-pyridyldithio)-ethylamine (PDA) to introduce disulfide bonds. The reversible capture and release of the protein reached an efficiency of 83.6% in release rate and could be repeated over 3 cycles within the microfluidic device. These results demonstrate that our redox-responsive hydrogel dots enable the dynamic capture and release of various different functionalized (macro)mols. (e.g., proteins and drugs) and have a great potential to be integrated into a lab-on-a-chip device for detection and/or delivery.
Polymers (Basel, Switzerland) published new progress about Bovine serum albumin Role: PEP (Physical, Engineering or Chemical Process), PUR (Purification or Recovery), PROC (Process), PREP (Preparation) (rhodamine B and PDA-labeled). 2127-03-9 belongs to class pyridine-derivatives, and the molecular formula is C10H8N2S2, Product Details of C10H8N2S2.