Iwata, Takahiro; Hirose, Hisaaki; Sakamoto, Kentarou; Hirai, Yusuke; Arafiles, Jan Vincent V.; Akishiba, Misao; Imanishi, Miki; Futaki, Shiroh published the artcile< Liquid Droplet Formation and Facile Cytosolic Translocation of IgG in the Presence of Attenuated Cationic Amphiphilic Lytic Peptides>, Formula: C10H8N2S2, the main research area is cervical cancer Fc binding ACAL peptide L17E IgG; antibodies; intracellular delivery; liquid droplet; liquid-liquid phase separation (LLPS); peptides.
Fc region binding peptide conjugated with attenuated cationic amphiphilic lytic peptide L17E trimer [FcB(L17E)3] was designed for IgG (IgG) delivery into cells. Particle-like liquid droplets were generated by mixing Alexa Fluor 488 labeled IgG (Alexa488-IgG) with FcB(L17E)3. Droplet contact with the cellular membrane led to spontaneous influx and distribution of Alexa488-IgG throughout cells in serum containing medium. Involvement of cellular machinery accompanied by actin polymerization and membrane ruffling was suggested for the translocation. Alexa488-IgG neg. charges were crucial in liquid droplet formation with pos. charged FcB(L17E)3. Binding of IgG to FcB(L17E)3 may not be necessary. Successful intracellular delivery of Alexa Fluor 594-labeled anti-nuclear pore complex antibody and anti-mCherry-nanobody tagged with supernegatively charged green fluorescence protein allowed binding to cellular targets in the presence of FcB(L17E)3.
Angewandte Chemie, International Edition published new progress about Blood serum. 2127-03-9 belongs to class pyridine-derivatives, and the molecular formula is C10H8N2S2, Formula: C10H8N2S2.