Hu, Yechen; Li, Yang; Gao, Hang; Jiang, Bo; Zhang, Xiaodan; Li, Xiao; Wu, Qiong; Liang, Zhen; Zhang, Lihua; Zhang, Yukui published the artcile< Cleavable hydrophobic derivatization strategy for enrichment and identification of phosphorylated lysine peptides>, Synthetic Route of 2127-03-9, the main research area is lysine phosohopeptide derivatization; Cleavable hydrophobic derivatization; Liquid chromatography–tandem mass spectrometry; N-Phosphorylation; Phosphorylated lysine peptides; Proteomics.
Because of structural flexibility and acid lability, the identification of phosphorylated lysine (pLys) peptides is a great challenge. The authors report here a cleavable hydrophobic derivatization (CHD) strategy for the enrichment and identification of pLys peptides. First, 2,5-dioxopyrrolidin-1-yl-3-(decyldithio)propanoate was synthesized to react with dephosphorylated lysine peptides, and then the derived peptides were captured by a C18 column, followed by cleavage of the hydrophobic chain, with the specific label left on the target peptides for further identification. By CHD, the enrichment of pLys peptides from interfering peptides (1:1000 mass ratio) was achieved. Furthermore, CHD was applied to screen the pLys targets from Escherichia coli lysates, and 39 pLys sites from 35 proteins were identified. Gene Ontol. (GO) anal. showed that these proteins played vital roles in catabolism, metabolism, biogenesis, and biosynthetic processes. All these results demonstrate that CHD might pave the way for comprehensive profiling of the pLys proteome.
Analytical and Bioanalytical Chemistry published new progress about Escherichia coli. 2127-03-9 belongs to class pyridine-derivatives, and the molecular formula is C10H8N2S2, Synthetic Route of 2127-03-9.