Esch, Patrick; Heiles, Sven published the artcile< Investigating C-C positions and hydroxylation sites in lipids using Paterno-Buechi functionalization mass spectrometry>, HPLC of Formula: 350-03-8, the main research area is double carbon bond lipid oxidation Paterno Buechi functionalization MS; Sacha inchi oil triglyceride oxidation Paterno Buechi functionalization MS.
Lipid oxidation plays a major role in biochem. processes and nutrition. Structural changes during oxidation can lead to alterations of lipid functions. Rancidification and production of secondary lipid messengers are well-known examples for the impact of oxidation on lipid function. Especially lipids with a high degree of unsaturation are prone to oxidize. In order to investigate structural changes of lipids upon oxidation, we here introduce a photochem. Paterno-Buechi functionalization workflow and subsequent mass spectrometric anal. for anal. of unsaturated, oxidized lipids. Results for hydroxylated fatty acids and triglycerides containing isolated and conjugated C-C bonds will be presented making use of 3-acetylpyridine as a photochem. active compound Photochem. derivatization is performed in nano-electrospray emitter tips in 30 s resulting in the formation of oxetanes without inducing light-triggered oxidation of analytes. Collisional-activation of photoproducts facilitates selective cleavage of oxetane moieties. Resulting fragment ions not only allow the determination of C-C bond locations for isolated and conjugated C-C bonds but also restrict the site of oxidation By registering the mass shift in some fragment ions of +15.99 Da due to hydroxylation, the oxidized sections of lipids can be identified. In order to demonstrate its anal. robustness, the method is applied to determine the structural impact of non-selective ambient oxidation on fatty acids, triglycerides and complex triglyceride mixtures obtained from Sacha inchi oil.
Analyst (Cambridge, United Kingdom) published new progress about C-C bond, double. 350-03-8 belongs to class pyridine-derivatives, and the molecular formula is C7H7NO, HPLC of Formula: 350-03-8.