Steyer, Daniel J.; Kennedy, Robert T. published the artcile< High-Throughput Nanoelectrospray Ionization-Mass Spectrometry Analysis of Microfluidic Droplet Samples>, Formula: C7H7NO, the main research area is high throughput nanoelectrospray mass spectrometry droplet microfluidics.
Droplet microfluidics enables high-throughput manipulation of fL-μL volume samples. Methods implemented for the chem. anal. of microfluidic droplets have been limited in scope, leaving some applications of droplet microfluidics difficult to perform or out of reach entirely. Nanoelectrospray ionization-mass spectrometry (nESI-MS) is an attractive approach for droplet anal., because it allows rapid, label-free, information-rich anal. with high mass sensitivity and resistance to matrix effects. Previous proof-of-concept systems for the nESI-MS anal. of droplets have been limited by the microfluidics used so that stable, long-term operation needed for high-throughput applications has not been demonstrated. The authors describe a platform for the stable anal. of microfluidic droplet samples by nESI-MS. Continuous infusion of droplets to an nESI emitter was demonstrated for as long as 2.5 h, corresponding to anal. of over 20 000 samples. Stable signal was observed for droplets as small as 65 pL and for throughputs as high as 10 droplets/s. A linear-concentration-based response and sample-to-sample carryover of <3% were also shown. The system is demonstrated for measuring products of in-droplet enzymic reactions. Analytical Chemistry (Washington, DC, United States) published new progress about Droplet microfluidics. 350-03-8 belongs to class pyridine-derivatives, and the molecular formula is C7H7NO, Formula: C7H7NO.