Mangala Prasad, Vidya; Leaman, Daniel P.; Lovendahl, Klaus N.; Croft, Jacob T.; Benhaim, Mark A.; Hodge, Edgar A.; Zwick, Michael B.; Lee, Kelly K. published the artcile< Cryo-ET of Env on intact HIV virions reveals structural variation and positioning on the Gag lattice>, Computed Properties of 2127-03-9, the main research area is HIV1 virions structural variation Env Gag glycosylation gp120; Gag-Env interaction; HIV Env glycoprotein; HIV assembly; broadly neutralizing antibody; cryo-electron microscopy; cryo-electron tomography; hydrogen/deuterium-exchange mass spectrometry; sub-tomogram averaging; vaccine design; virus structure.
HIV-1 Env mediates viral entry into host cells and is the sole target for neutralizing antibodies. However, Env structure and organization in its native virion context has eluded detailed characterization. Here, we used cryo-electron tomog. to analyze Env in mature and immature HIV-1 particles. Immature particles showed distinct Env positioning relative to the underlying Gag lattice, providing insights into long-standing questions about Env incorporation. A 9.1-Å sub-tomogram-averaged reconstruction of virion-bound Env in conjunction with structural mass spectrometry revealed unexpected features, including a variable central core of the gp41 subunit, heterogeneous glycosylation between protomers, and a flexible stalk that allows Env tilting and variable exposure of neutralizing epitopes. Together, our results provide an integrative understanding of HIV assembly and structural variation in Env antigen presentation.
Cell (Cambridge, MA, United States) published new progress about Antibodies and Immunoglobulins Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 2127-03-9 belongs to class pyridine-derivatives, and the molecular formula is C10H8N2S2, Computed Properties of 2127-03-9.