《Bis(zinc(II)-dipicolylamine)-functionalized sub-2 μm core-shell microspheres for the analysis of N-phosphoproteome》 was published in Nature Communications in 2020. These research results belong to Hu, Yechen; Jiang, Bo; Weng, Yejing; Sui, Zhigang; Zhao, Baofeng; Chen, Yuanbo; Liu, Lukuan; Wu, Qiong; Liang, Zhen; Zhang, Lihua; Zhang, Yukui. Product Details of 1539-42-0 The article mentions the following:
Protein N-phosphorylation plays a critical role in central metabolism and two/multicomponent signaling of prokaryotes. However, the current enrichment methods for O-phosphopeptides are not preferred for N-phosphopeptides due to the intrinsic lability of P-N bond under acidic conditions. Therefore, the effective N-phosphoproteome anal. remains challenging. Herein, bis(zinc(II)-dipicolylamine)-functionalized sub-2 μm core-shell silica microspheres (SiO2@DpaZn) are tailored for rapid and effective N-phosphopeptides enrichment. Due to the coordination of phosphate groups to Zn(II), N-phosphopeptides can be effectively captured under neutral conditions. Moreover, the method is successfully applied to an E.coli and HeLa N-phosphoproteome study. These results further broaden the range of methods for the discovery of N-phosphoproteins with significant biol. functions. In the experiment, the researchers used many compounds, for example, Bis(pyridin-2-ylmethyl)amine(cas: 1539-42-0Product Details of 1539-42-0)
Bis(pyridin-2-ylmethyl)amine(cas: 1539-42-0) is a secondary amine with two picolyl substituents. As a tridentate ligand this compound provides three nitrogen donors that affords good selectivity for Zn2+ over biologically relevant metals such as Na+, K+, Mg2+ and Ca2+, and leaves coordination sites free for anion binding. Product Details of 1539-42-0