ãSensitive Detection of Broad-Spectrum Bacteria with Small-Molecule Fluorescent Excimer Chemosensorsã?was published in ACS Sensors in 2020. These research results belong to Cabral, Aaron D.; Rafiei, Nafiseh; de Araujo, Elvin D.; Radu, Tudor B.; Toutah, Krimo; Nino, Daniel; Murcar-Evans, Bronte I.; Milstein, Joshua N.; Kraskouskaya, Dziyana; Gunning, Patrick T.. HPLC of Formula: 1539-42-0 The article mentions the following:
Antibiotic resistance is a major problem for world health, triggered by the unnecessary usage of broad-spectrum antibiotics on purportedly infected patients. Current clin. standards require lengthy protocols for the detection of bacterial species in sterile physiol. fluids. A class of small-mol. fluorescent chemosensors termed ProxyPhos is capable of rapid, sensitive, and facile detection of broad-spectrum bacteria. The sensors act via a turn-on fluorescent excimer mechanism, where close-proximity binding of multiple sensor units amplifies a red shift emission signal. ProxyPhos sensors were able to detect down to 10 CFUs of model strains by flow cytometry assays and showed selectivity over mammalian cells in a bacterial coculture through fluorescence microscopy. The studies reveal that the zinc(II)-chelates cyclen and cyclam are novel and effective binding units for the detection of both Gram-neg. and Gram-pos. bacterial strains. Mode of action studies revealed that the chemosensors detect Gram-neg. and Gram-pos. strains with two distinct mechanisms. Preliminary studies applying ProxyPhos sensors to sterile physiol. fluids (cerebrospinal fluid) in flow cytometry assays were successful. The results suggest that ProxyPhos sensors can be developed as a rapid, inexpensive, and robust tool for the “”yes-no”” detection of broad-spectrum bacteria in sterile fluids. In the experiment, the researchers used many compounds, for example, Bis(pyridin-2-ylmethyl)amine(cas: 1539-42-0HPLC of Formula: 1539-42-0)
Bis(pyridin-2-ylmethyl)amine(cas: 1539-42-0) is a secondary amine with two picolyl substituents. As a tridentate ligand this compound provides three nitrogen donors that affords good selectivity for Zn2+ over biologically relevant metals such as Na+, K+, Mg2+ and Ca2+, and leaves coordination sites free for anion binding. HPLC of Formula: 1539-42-0