《Phosphoprotein Detection with a Single Nanofluidic Diode Decorated with Zinc Chelates》 was published in ChemPlusChem in 2020. These research results belong to Nasir, Saima; Ali, Mubarak; Ahmed, Ishtiaq; Niemeyer, Christof M.; Ensinger, Wolfgang. Category: pyridine-derivatives The article mentions the following:
We report a nanofluidic device for the label-free detection of phosphoprotein (PPn) analytes. To achieve this goal, a metal ion chelator, namely 4-[bis(2-pyridylmethyl)aminomethyl]aniline (DPA-NH2) compound was synthesized. Single asym. nanofluidic channels were fabricated in polyethylene terephthalate (PET) membranes. The chelator (DPA-NH2) mols. are subsequently immobilized on the nanochannel surface, followed by the zinc ion complexation to afford DPA-Zn2+ chelates, which act as ligand moieties for the specific binding of phosphoproteins. The success of the chem. reaction and biomol. recognition process that occur in a confined geometry can be monitored from the changes in elec. readout of the nanochannel. The nanofluidic sensor has the ability to sensitively and specifically detect lower concentrations (≥1 nM) of phosphoprotein (albumin and α-casein) in the surrounding environment as evidenced from the significant decrease in ion current flowing through the nanochannels. However, dephosphoproteins such as lysozyme and dephospho-α-casein even at higher concentration (>1μM) could not induce any significant change in the transmembrane ion flux. This observation indicated the sensitivity and specificity of the proposed nanofluidic sensor towards PPn proteins, and has potential for use in differentiating between phosphoproteins and dephosphoproteins. The results came from multiple reactions, including the reaction of Bis(pyridin-2-ylmethyl)amine(cas: 1539-42-0Category: pyridine-derivatives)
Bis(pyridin-2-ylmethyl)amine(cas: 1539-42-0) is a secondary amine with two picolyl substituents. As a tridentate ligand this compound provides three nitrogen donors that affords good selectivity for Zn2+ over biologically relevant metals such as Na+, K+, Mg2+ and Ca2+, and leaves coordination sites free for anion binding. Category: pyridine-derivatives