(4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7) belongs to pyridine derivatives. Pyridine’s the lone pair does not contribute to the aromatic system but importantly influences the chemical properties of pyridine, as it easily supports bond formation via an electrophilic attack. Several pyridine derivatives play important roles in biological systems. While its biosynthesis is not fully understood, nicotinic acid (vitamin B3) occurs in some bacteria, fungi, and mammals.Quality Control of (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate
Characterization and application of L-methionine γ-lyase Q349S mutant enzyme with an enhanced activity toward L-homocysteine was written by Okawa, Atsushi;Handa, Haruhisa;Yasuda, Eri;Murota, Masaki;Kudo, Daizo;Tamura, Takashi;Shiba, Tomoo;Inagaki, Kenji. And the article was included in Journal of Bioscience and Bioengineering in 2022.Quality Control of (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate The following contents are mentioned in the article:
L-Methionine γ-lyse (MGL), a pyridoxal 5′-phosphate-dependent enzyme, catalyzes the α,γ-elimination of L-methionine (L-Met) and L-homocysteine (L-Hcy) to produce α-keto acids, thiols, and ammonia. Previously, various mutant enzymes of Pseudomonas putida MGL (PpMGL) were prepared to identify a homocysteine (Hcy)-specific enzyme that would assist the diagnosis of homocystinuria. Among the mutat enzymes the Q349S mutant exhibited high degradation activity toward L-Hcy. In the present study, PpMGL Q349S was characterized; the results suggested that it could be applied to determine the amount of L-Hcy. Compared to the wild-type PpMGL, specific activities of the Q349S mutant with L-Hcy and L-Met were 1.5 and 0.7 times, resp. Addnl., we confirmed that L-Hcy in plasma samples could be accurately detected using the Q349S mutant by preincubating it with cysteine desulfurase (CsdA). Furthermore, we determined the X-ray crystal structure of PpMGL Q349S L-Met or L-Hcy complexes Michaelis complex, germinal diamine, and external aldimine at 2.25-2.40 Å. These 3D structures showed that the interaction partner of the β-hydroxyl group of Thr355 in the wild-type PpMGL was changed to the carboxyl group of the Hcy-PLP external aldimine in the Q349S mutant. The interaction of Ser349 and Arg375 was different between L-Met and L-Hcy recognition, indicating that it was important for the recognition of the carboxyl group of the substrate. This study involved multiple reactions and reactants, such as (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7Quality Control of (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate).
(4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7) belongs to pyridine derivatives. Pyridine’s the lone pair does not contribute to the aromatic system but importantly influences the chemical properties of pyridine, as it easily supports bond formation via an electrophilic attack. Several pyridine derivatives play important roles in biological systems. While its biosynthesis is not fully understood, nicotinic acid (vitamin B3) occurs in some bacteria, fungi, and mammals.Quality Control of (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate
Referemce:
Pyridine – Wikipedia,
Pyridine | C5H5N – PubChem