Transcriptome analysis of the effect of high-temperature on nutrient metabolism in juvenile grass carp (Ctenopharyngodon idellus) was written by Huang, Dongyu;Ren, Mingchun;Liang, Hualiang;Ge, Xianping;Xu, Hao;Wu, Longhua. And the article was included in Gene in 2022.HPLC of Formula: 54-47-7 The following contents are mentioned in the article:
To investigate the variations in gene expression in grass carp under high-temperature stress, two libraries were constructed from a high-temperature treatment group (T33) and a control group (T27) and sequenced using Illumina sequencing technol. The results showed that sequencing generated a total of 279,398,348 raw reads, approx. 40.7-51.8 M clean reads were obtained from each library, and the percentage of uniquely mapped transcripts ranged from 80.13 to 84.58%. A total of 260 differentially expressed genes (DEGs) were identified under high-temperature stress, among which 84 genes were upregulated and 176 genes were downregulated. Ten DEGs were randomly selected for quant. RT-PCR (qRT-PCR) anal., and the results confirmed that the transcriptome anal. was reliable. Furthermore, the DEGs were subjected to Gene Ontol. (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment anal., and the results showed that most of the DEGs were involved in protein, lipid and carbohydrate metabolism Moreover, plasma urea nitrogen (Urea) and triglyceride (TG) contents were significantly lower in the high-temperature treatment group than in the control group (P < 0.01). In summary, these results indicated that high-temperature stress could inhibit protein synthesis, decrease fatty acid synthesis, and weaken carbohydrate metabolism in juvenile grass carp. This study involved multiple reactions and reactants, such as (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7HPLC of Formula: 54-47-7).
(4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7) belongs to pyridine derivatives. In contrast to benzene, Pyridine’s electron density is not evenly distributed over the ring, reflecting the negative inductive effect of the nitrogen atom. Pyridine derivatives are also useful as small-molecule α-helix mimetics that inhibit protein-protein interactions, as well as functionally selective GABA ligands.HPLC of Formula: 54-47-7