Gene set enrichment analysis and ingenuity pathway analysis to identify biomarkers in Sheng-ji Hua-yu formula treated diabetic ulcers was written by Ru, Yi;Zhang, Ying;Xiang, Yan-wei;Luo, Ying;Luo, Yue;Jiang, Jing-si;Song, Jian-kun;Fei, Xiao-ya;Yang, Dan;Zhang, Zhan;Zhang, Hui-ping;Liu, Tai-yi;Yin, Shuang-yi;Li, Bin;Kuai, Le. And the article was included in Journal of Ethnopharmacology in 2022.Safety of (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate The following contents are mentioned in the article:
Sheng-ji Hua-yu (SJHY) formula is a Chinese herbal prescription for diabetic ulcers (DUs) treatment, which can accelerate wound reconstruction and shorten the healing time. However, its mechanism role maintains unclear. To elucidate the mol. mechanisms of SJHY application on DUs. To begin with, transcriptome sequencing was adopted to identified differentially expression mRNAs among normal ulcers, DUs, and DUs + SJHY treatment in vivo. Liquid chromatog.-tandem mass spectrometry was applied for the quality control of SJHY formula. GO and KEGG enrichment anal. were used to identify the mechanisms underlying the therapeutic effect of SJHY formula, and then gene set enrichment anal. and ingenuity pathway anal. were conducted for functional anal. Further, qPCR detection was performed in vivo for validation. SJHY administration could regulate the glucose metabolic process, AMPK and HIF-1 pathway to accelerate healing processes of DUs. Besides, CRHR1, SHH, and GAL were identified as the critical targets, and SLC6A3, GRP, FGF23, and CYP27B1 were considered as the upstream genes of SJHY treatment. Combined with animal experiments, the prediction results were validated in DUs mice model. This study used modular pharmacol. anal. to identify the biomarkers of SJHY formula and provide the potential therapeutic targets for DUs treatment as well. This study involved multiple reactions and reactants, such as (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7Safety of (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate).
(4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7) belongs to pyridine derivatives. Pyridine is diamagnetic and has a diamagnetic susceptibility of â?8.7 à 10â? cm3·molâ?.The molecular electric dipole moment is 2.2 debyes. The standard enthalpy of formation is 100.2 kJ·molâ? in the liquid phase and 140.4 kJ·molâ? in the gas phase. Many analogues of pyridine are known where N is replaced by other heteroatoms . Substitution of one CâH in pyridine with a second N gives rise to the diazine heterocycles (C4H4N2), with the names pyridazine, pyrimidine, and pyrazine.Safety of (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate