Expression, purification, and characterization of glutamate decarboxylase from human gut-originated Lactococcus garvieae MJF010 was written by Lim, Hyo Jung;Jung, Dong-Hyun;Cho, Eui-Sang;Seo, Myung-Ji. And the article was included in World Journal of Microbiology & Biotechnology in 2022.COA of Formula: C8H10NO6P The following contents are mentioned in the article:
Human gut-originated lactic acid bacteria were cultivated, and high 纬-aminobutyric acid (GABA)-producing Lactococcus garvieae MJF010 was identified. To date, despite the importance of GABA, no studies have investigated GABA-producing Lactococcus species, except for Lc. lactis. A recombinant glutamate decarboxylase of the strain MJF010 (rLgGad) was successfully expressed in Escherichia coli BL21(DE3) with a size of 53.9 kDa. rLgGad could produce GABA, which was verified using the silylation-derivative fragment ions of GABA. The purified rLgGad showed the highest GABA-producing activity at 35掳C and pH 5. rLgGad showed a melting temperature of 43.84掳C. At 30掳C, more than 80% of the activity was maintained even after 7 h; however, it rapidly decreased at 50掳C. The kinetic parameters, Km, Vmax, and kcat, of rLgGad were 2.94 mM, 0.023 mM/min, and 12.3 min- 1, resp. The metal reagents of CaCl2, MgCl2, and ZnCl2 significantly had pos. effects on rLgGad activity. However, most coenzymes including pyridoxal 5-phosphate showed no significant effects on enzyme activity. In conclusion, this is the first report of Gad from Lc. garvieae species and provides important enzymic information related to GABA biosynthesis in the Lactococcus genus. This study involved multiple reactions and reactants, such as (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7COA of Formula: C8H10NO6P).
(4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7) belongs to pyridine derivatives. Pyridines are an important class of heterocycles and occur in polysubstituted forms in many naturally occurring biologically active compounds, drug molecules and chiral ligands. Pyridine derivatives are also useful as small-molecule 伪-helix mimetics that inhibit protein-protein interactions, as well as functionally selective GABA ligands.COA of Formula: C8H10NO6P