Design and fabrication of reusable core-shell composite microspheres based on nanodiamond for selective enrichment of phosphopeptides was written by Jia, Shicong;Tang, Ruizhi;Zhang, Shuai;Gao, Zheng;Gong, Bolin;Ma, Shujuan;Ou, Junjie. And the article was included in Microchimica Acta in 2022.HPLC of Formula: 54-47-7 The following contents are mentioned in the article:
A kind of core-shell composite microsphere (CM) with nano-on-micro structure was synthesized via grafting amine-modified nanodiamonds onto the surface of monodisperse nonporous polymeric microsphere. In this way, the agglomeration of nanodiamond particles in the solution was avoided. After modification with pyrogallol groups, CM could chelate titanium ions (Ti4+) and thus be utilized as immobilized metal affinity chromatog. (IMAC) sorbent to enrich phosphopeptides from biol. samples. The resulting Ti4+-CM exhibited high enrichment efficiency and specificity to phosphopeptides. A total of 106 of unique phosphopeptides mapped to 29 phosphoproteins were clearly identified from 5渭L of a milk digest after enrichment. Owing to the strong chelation between Ti4+ and pyrogallol ligands, the Ti4+ is not released from the sorbent after completion of the enrichment process. As a result, the Ti4+-CM sorbent could be reused, and no significant loss of enrichment efficiency occurred even on the fourth run employing a 尾-casein digest as the sample. The strategy adopted presents a new way to prepare a high-performance reusable IMAC sorbent. This study involved multiple reactions and reactants, such as (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7HPLC of Formula: 54-47-7).
(4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7) belongs to pyridine derivatives. In contrast to benzene, Pyridine’s electron density is not evenly distributed over the ring, reflecting the negative inductive effect of the nitrogen atom. Pyridine groups exist in countless molecules, and their applications include catalysis, drug design, molecular recognition, and natural product synthesis.HPLC of Formula: 54-47-7