Zarybnicky, Tomas published the artcileThe selection and validation of reference genes for mRNA and microRNA expression studies in human liver slices using RT-qPCR, Product Details of C18H19Cl2NO4, the main research area is insulin YWHAZ ACTB B2M CYP3A4 CYP1A2 miR165p biomarker steatosis; RT-qPCR; human liver; mRNA; miRNA; precision-cut liver slices; reference gene.
The selection of a suitable combination of reference genes (RGs) for data normalization is a crucial step for obtaining reliable and reproducible results from transcriptional response anal. using a reverse transcription-quant. polymerase chain reaction. This is especially so if a three-dimensional multicellular model prepared from liver tissues originating from biol. diverse human individuals is used. The mRNA and miRNA RGs stability were studied in thirty-five human liver tissue samples and twelve precision-cut human liver slices (PCLS) treated for 24 h with DMSO (controls) and PCLS treated with β-naphthoflavone (10μM) or rifampicin (10μM) as cytochrome P 450 (CYP) inducers. Validation of RGs was performed by an expression anal. of CYP3A4 and CYP1A2 on rifampicin and β-naphthoflavone induction, resp. Regarding mRNA, the best combination of RGs for the controls was YWHAZ and B2M, while YWHAZ and ACTB were selected for the liver samples and treated PCLS. Stability of all candidate miRNA RGs was comparable or better than that of generally used short non-coding RNA U6. The best combination for the control PCLS was miR-16-5p and miR-152-3p, in contrast to the miR-16-5b and miR-23b-3p selected for the treated PCLS. Our results showed that the candidate RGs were rather stable, especially for miRNA in human PCLS.
Genes published new progress about Biomarkers. 72509-76-3 belongs to class pyridine-derivatives, name is 3-Ethyl 5-methyl 4-(2,3-dichlorophenyl)-2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate, and the molecular formula is C18H19Cl2NO4, Product Details of C18H19Cl2NO4.