Salunkhe, N. H. published the artcileValidated RP-HPLC method for quantification of felodipine in rabbit plasma: Application in a bioequivalence study, Name: 3-Ethyl 5-methyl 4-(2,3-dichlorophenyl)-2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate, the main research area is RP HPLC method felodipine rabbit plasma; Felodipine; La nimodipine; Nimodipine; Plasma de lapin; RP-HPLC; Rabbit plasma; Validation.
The aim of present study was to develop a simple, rapid, selective, sensitive and robust reverse phase high performance liquid chromatog. method for quantification of felodipine in rabbit plasma at the wavelength of 360 nm. Protein was precipitated from rabbit plasma sample by addition of acetonitrile as a vehicle. An isocratic elution of samples was performed on capcell pak C8 DD S5 column (4.6 mm × 250 mm particle size 5μm) column with mobile phase consisting 5 mM Phosphate Buffer (pH 4.8 adjusted with dilute ortho-phosphoric acid solution): acetonitrile (25:75:volume/volume) delivered at flow rate 1.0 mL min-1. A good linear response was achieved over the range of 0.25-20.00 g mL-1. LODs and LOQs for felodipine were found to be 0.055 and 0.201μg mL-1, resp. The method was quant. evaluated in terms of linearity, precision, accuracy (recovery), selectivity robustness and stability study as per standard guidelines. The validated RP-HPLC method was successfully applied for the bioavailability studies of felodipine. The pharmacokinetic parameters were calculated for all the investigated drugs in rabbit after single-dose administrations of pure drug and formulation of felodipine. Finally, the obtained results for the application of the proposed RP-HPLC method proved its efficiency to be applied to the therapeutic drug monitoring (TDM) and bioequivalence (BE) studies. Thus, developed method is simple, convenient and suitable for the anal. of felodipine in bulk and pharmaceutical formulations.
Annales Pharmaceutiques Francaises published new progress about Blood plasma. 72509-76-3 belongs to class pyridine-derivatives, name is 3-Ethyl 5-methyl 4-(2,3-dichlorophenyl)-2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate, and the molecular formula is C18H19Cl2NO4, Name: 3-Ethyl 5-methyl 4-(2,3-dichlorophenyl)-2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate.