Dzodic, Predrag published the artcileA reliable chromatographic method for the simultaneous determination of ciprofloxacin and moxifloxacin in human serum, Synthetic Route of 72509-76-3, the main research area is serum ciprofloxacin moxifloxacin high performance liquid chromatog.
Purpose: To develop and validate a simple chromatog. method for the anal. of ciprofloxacin and moxifloxacin in human serum. Methods: After protein precipitation had been performed, high performance liquid chromatog. (HPLC) with UV detection was utilized for the anal. of ciprofloxacin and moxifloxacin in human serum. Anal. column Zorbax SB-C18 (150 mm x 4.6 mm i.d., particle size 3.5μm) was used as a stationary phase. Chromatog. separation was realized with the mobile phase 0.1% trifluoroacetic acid in water for chromatog. – methanol (66:34, volume/volume), at the flow rate of 1 mL/min, temperature of 35°C and detection at 280 nm. The method validation was performed according to the guidelines of the European Medicines Agency (EMA). Results: The chromatog. run time was about 12 min and no interference was observed For ciprofloxacin, the method was linear over a concentration range of 0.5-50μg/mL, with a correlation coefficient of 0.9874. For moxifloxacin, the method was linear over a concentration range of 0.5-50μg/mL, with a correlation coefficient of 0.9946. Since relative standard deviation (RSD) and relative recovery values were within acceptable limits according to EMA guidelines, good intra-day precision, inter-day precision, as well as the accuracy of the method, were observed Conclusion: A simple and reliable HPLC-UV method has been developed and validated for the simultaneous determination of ciprofloxacin and moxifloxacin in human serum. The method can be applied for therapeutic drug monitoring but also and pharmacokinetic studies of ciprofloxacin and moxifloxacin.
Tropical Journal of Pharmaceutical Research published new progress about Blood serum. 72509-76-3 belongs to class pyridine-derivatives, name is 3-Ethyl 5-methyl 4-(2,3-dichlorophenyl)-2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate, and the molecular formula is C18H19Cl2NO4, Synthetic Route of 72509-76-3.