Bayer, Robin published the artcileMechano-pharmacological testing of L-type Ca2+ channel modulators via human vascular celldrum model, Application In Synthesis of 21829-25-4, the main research area is verapamil antioxidant calcium channel agonist smooth muscle cell stress; Celldrum; Biomechanics; Mechanobiology; Human arterial smooth muscle cells (haSMC); Vasoconstriction; Vasopressors; Vasoactive agents.
This study aimed to establish a precise and well-defined working model, assessing pharmaceutical effects on vascular smooth muscle cell monolayer in-vitro. This study focuses on the dilative and contractive effects of L-type Ca2+ channel agonists and antagonists, resp. We analyzed the effects of Bay K8644, nifedipine and verapamil. Three different measurement modes were developed and applied to determine the most appropriate anal. technique for the study purpose. These three operation modes are called, particular time mode, long term mode and real-time mode. It was possible to quantify the biomech. response of haSMCs to the addition of vasoactive agents using CellDrum technol. Due to the supplementation of 100nM Bay K8644, the tension increased approx. 10.6% from initial tension maximum, whereas, the treatment with nifedipine and verapamil caused a significant decrease in cellular tension: 10nM nifedipine decreased the biomech. stress around 6,5% and 50nM verapamil by 2,8%, compared to the initial tension maximum Addnl., all tested measurement modes provide similar results while focusing on different anal. parameters. Conclusion: The CellDrum technol. allows highly sensitive biomech. stress measurements of cultured haSMC monolayers. The mech. stress responses evoked by the application of vasoactive calcium channel modulators were quantified functionally (N/m2). All tested operation modes resulted in equal findings, whereas each mode features operation-related data anal.
Cellular Physiology & Biochemistry published new progress about Apoptosis. 21829-25-4 belongs to class pyridine-derivatives, name is Dimethyl 2,6-dimethyl-4-(2-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate, and the molecular formula is C17H18N2O6, Application In Synthesis of 21829-25-4.