Meso-pyridinium substituted BODIPY dyes as mitochondria-targeted probes for the detection of cysteine in living cells and in vivo was written by Ji, Xin;Wang, Nannan;Zhang, Jian;Xu, Shuang;Si, Yubing;Zhao, Weili. And the article was included in Dyes and Pigments in 2021.Synthetic Route of C5H5NO This article mentions the following:
Cysteine (Cys) is a crucial bio-thiol attributing to its significant role in many physiol. and pathol. processes and maintaining redox dynamic balance. In mitochondria, ROS can cause oxidative damage, and Cys can solve this problem by acting as an antioxidant. Herein, we designed and synthesized a series of meso-pyridin and meso-pyridinium substituted BODIPY dyes as fluorescent probes to distinguish Cys. Through screening the responsive behaviors of the all probes toward biothiols, we obtained an appropriate mitochondria-targeted probe BDP-S-o-Py+ for Cys-specific detection. D. functional theory (DFT) calculations were used to demonstrate the different reactivity of the probes. BDP-S-o-Py+ could detect Cys with fast response under biol. environment. The probe BDP-S-o-Py+ had a low limit of detection (LOD, 72 nM) at the linear dynamic range from 0 to 50 μM. In particularly, BDP-S-o-Py+ could be used for imaging of mitochondrial Cys in living cells, and was capable of monitoring endogenous Cys in vivo, successfully. In the experiment, the researchers used many compounds, for example, Pyridin-4-ol (cas: 626-64-2Synthetic Route of C5H5NO).
Pyridin-4-ol (cas: 626-64-2) belongs to pyridine derivatives. Pyridines are an important class of heterocycles and occur in polysubstituted forms in many naturally occurring biologically active compounds, drug molecules and chiral ligands. Pyridine groups exist in countless molecules, and their applications include catalysis, drug design, molecular recognition, and natural product synthesis.Synthetic Route of C5H5NO