Quantitation of genetox impurities using a surrogate standard approach was written by Wang, Heather;Nardi, Regina;Bereznitski, Yuri;Helmy, Roy;Waterhouse, David J.. And the article was included in Journal of Analytical Sciences, Methods and Instrumentation in 2013.Recommanded Product: 3-Hydroxy-6-methyl-2-nitropyridine This article mentions the following:
With the ever increasing complexity of active pharmaceutical ingredient (API) preparations, more potential genotoxic impurities (PGI’s) are being observed It is thus necessary to determine if these PGI’s are present in the final API’s, and if they are present, to ensure the levels are acceptable for any clin. uses. For PGI’s that have authentic standards available, quantitation can be accomplished in a straightforward manner. However, for PGI’s that are expected to form through rearrangements or side reactions, authentic standards may not be readily available, significantly complicating the anal. In this study we describe a surrogate standard approach for quantifying PGI’s that allows for relative response factor calculations of PGI species utilizing both gas chromatog.-mass spectrometry (GC-MS) and liquid chromatog.-mass spectrometry (LC-MS). In the experiment, the researchers used many compounds, for example, 3-Hydroxy-6-methyl-2-nitropyridine (cas: 15128-90-2Recommanded Product: 3-Hydroxy-6-methyl-2-nitropyridine).
3-Hydroxy-6-methyl-2-nitropyridine (cas: 15128-90-2) belongs to pyridine derivatives. Pyridine’s the lone pair does not contribute to the aromatic system but importantly influences the chemical properties of pyridine, as it easily supports bond formation via an electrophilic attack. Many analogues of pyridine are known where N is replaced by other heteroatoms . Substitution of one C–H in pyridine with a second N gives rise to the diazine heterocycles (C4H4N2), with the names pyridazine, pyrimidine, and pyrazine.Recommanded Product: 3-Hydroxy-6-methyl-2-nitropyridine