Azadioxatriangulenium: Synthesis and Photophysical Properties of Reactive Dyes for Bioconjugation was written by Bora, Ilkay;Bogh, Sidsel A.;Santella, Marco;Rosenberg, Martin;Sorensen, Thomas Just;Laursen, Bo W.. And the article was included in European Journal of Organic Chemistry in 2015.Name: Pyridinehydrochloride This article mentions the following:
Azadioxatriangulenium (ADOTA) is a fluorescent triangulenium dye with a long fluorescence lifetime, highly polarized transitions and emission in the red part of the visible spectrum. These properties make the chromophore suited for application in fluorescence polarization/anisotropy assays. To be useful for these applications, reactive forms of the dyes must be available in significant quantities. Here, the synthesis and photophys. properties of amine-reactive N-hydroxysuccinimide (NHS) esters and a thiol-reactive maleimide derivative of ADOTA are reported. The synthesis involves two steps of nucleophilic bridge-forming reactions starting from tris(2,6-dimethoxyphenyl)methylium tetrafluoroborate, which can readily be prepared on a 100 g scale. In the third and final step the reactive NHS or maleimide groups are formed. The beneficial photophys. properties of the ADOTA chromophore are maintained in these derivatives, and we conclude that these systems are ideal to study protein motion and protein-protein interactions for systems of up to 1000 kDa. In the experiment, the researchers used many compounds, for example, Pyridinehydrochloride (cas: 628-13-7Name: Pyridinehydrochloride).
Pyridinehydrochloride (cas: 628-13-7) belongs to pyridine derivatives. Pyridine has a conjugated system of six π electrons that are delocalized over the ring. The molecule is planar and, thus, follows the Hückel criteria for aromatic systems. Pyridine, its benzo and pyridine-based compounds play diverse roles in organic chemistry. Pyridine-based materials are valued for their optical and physical properties as well as their medical potential. Name: Pyridinehydrochloride