Electric Literature of 116355-16-9, Researchers who often do experiments know that organic synthesis is a process of preparing more complex target molecules from simple raw materials through one or more chemical reactions. Generally, it requires fewer steps,and cheap raw materials. 116355-16-9, name is Imidazo[1,2-a]pyridine-6-carbaldehyde. A new synthetic method of this compound is introduced below.
General procedure: To a 2mL Eppendorf tube was added 10muL of the aldehyde stock solution in DMSO (0.1M, final concentration 10mM), 90muL of buffer (100mM phosphate buffer, 0.1mM PLP, 2M glycine, pH=7.0) and 2muL of a 10.16mg/mL enzyme solution in phosphate buffer (100mM, 1mM PLP, pH=7.0, 0.2mg/mL final protein concentration). The mixture was incubated at 40C and 1100rpm on an Eppendorf Thermomixer. After 1.5h, the reaction was quenched with 200muL ACN/MeOH (1:1), stirred for 5min at 1100rpm and centrifuged at 21,130 xg for 3min. The clear supernatant was analyzed using an UPLC monitoring method (see supplementary information).
At the same time, in my other blogs, there are other synthetic methods of this type of compound,116355-16-9, Imidazo[1,2-a]pyridine-6-carbaldehyde, and friends who are interested can also refer to it.
Reference:
Article; Ligibel, Mathieu; Moore, Charles; Bruccoleri, Robert; Snajdrova, Radka; Biochimica et Biophysica Acta – Proteins and Proteomics; vol. 1868; 2; (2020);,
Pyridine – Wikipedia,
Pyridine | C5H5N – PubChem