I found the field of Engineering very interesting. Saw the article An L-threonine aldolase for asymmetric synthesis of beta-hydroxy-alpha-amino acids published in 2020.0. Recommanded Product: 500-22-1, Reprint Addresses Lin, J (corresponding author), Fuzhou Univ, Coll Chem Engn, Fuzhou 350116, Peoples R China.. The CAS is 500-22-1. Through research, I have a further understanding and discovery of 3-Pyridinecarboxaldehyde
L-threonine aldolase (LTA) is a PLP-dependent enzyme that can reversibly catalyze aldol reaction of glycine and acetaldehyde to produce beta-hydroxy-alpha-amino acids. In the present work, a putative Ita gene from Actinocorallia herbida (AhLTA) was mined and over-expressed in Escherichia coli BL21 (DE3). The substrate spectrum assay indicated that AhLTA only used glycine as donor substrate and tolerated a wild range of aromatic aldehydes as acceptor substrates. It was found that the type and position of substituents in the aromatic aldehydes exerted a significant impact on the activity and stereoselectivity at beta-carbon of AhLTA. Among those substrates, AhLTA could catalyze glycine and 4-methylsulphonyl benzaldehyde (14a) to produce L-threo-4-methylsulfonylphenylserine ((2S,3R)-14b) with high conversion (94.4%) and moderate stereoselectivity (19% de). By conditional optimization, the de value of (2S, 3R)-14b was improved to 61% and the conversion was 75%. Taken together, our study suggested that AhLTA might be a promising catalyst for producing chiral beta-hydroxy-alpha-amino acids. (C) 2020 Elsevier Ltd. All rights reserved.
Recommanded Product: 500-22-1. About 3-Pyridinecarboxaldehyde, If you have any questions, you can contact Wang, LC; Xu, L; Xu, XQ; Su, BM; Lin, J or concate me.
Reference:
Pyridine – Wikipedia,
,Pyridine | C5H5N – PubChem