Aramesh-Boroujeni, Zahra; Jahani, Shohreh; Khorasani-Motlagh, Mozhgan; Kerman, Kagan; Noroozifar, Meissam published the artcile< Evaluation of DNA, BSA binding, DNA cleavage and antimicrobial activity of ytterbium(III) complex containing 2,2′-bipyridine ligand>, Product Details of C10H8N2, the main research area is albumin DNA ytterbium antimicrobial activity; Antibacterial activity; binding interaction; bovine serum albumin; fish salmon-DNA; molecular docking; ytterbium(III) complex.
In order to estimate the biol. potential of a synthesized complex [Yb(bpy)2Cl3.OH2] where bpy is 2,2′-bipyridine, its binding behavior with fish salmon-DNA and bovine serum albumin were studied by different kinds of spectroscopy and mol. modeling methods. This complex was selected for its antibacterial and antifungal activities as well as the DNA cleavage activities were examined by agarose gel electrophoresis. The analyses of fluorescence data at four temperatures were done in order to evaluate the binding and thermodn. parameters of the interaction of Yb(III) complex with DNA and BSA. The exptl. results indicated that the major binding modes were based on groove binding with DNA and BSA. In addition, iodide quenching studies, ethidium bromide (EtBr) exclusion assay, ionic strength effect, CD, and viscosity studies reflected the binding of Yb(III) complex explicitly with the FS-DNA mainly in a groove binding mode. Moreover, mol. docking studies indicated that this complex was bound to the minor groove of DNA and to polar and apolar residues located in the subdomain IB of BSA (site 3). Also, the results of competitive experiments assessed site 3 of BSA as the most probable binding site for this complex. The mol. docking results were in good agreement with our exptl. results. From both exptl. and docking results, the binding constant values displayed the remarkably high affinity of Yb(III) complex to DNA as well as BSA.Communicated by Ramaswamy H. Sarma
Journal of Biomolecular Structure and Dynamics published new progress about Acinetobacter baumannii. 366-18-7 belongs to class pyridine-derivatives, and the molecular formula is C10H8N2, Product Details of C10H8N2.