Banda, Jagadeesh; Lakshmanan, Ramalingam; Vvs, Shiva Prasad; Gudla, Srinibas Patro; Prudhivi, Rosaiah published an article in 2015, the title of the article was A highly sensitive method for the quantification of fludrocortisone in human plasma using ultra-high-performance liquid chromatography tandem mass spectrometry and its pharmacokinetic application.Application of 132-20-7 And the article contains the following content:
A simple and high sensitive ultra-high-performance liquid chromatog. tandem mass spectrometry method for the determination of fludrocortisone in human plasma was developed and validated as per guidelines. The analyte and internal standard (IS), fludrocortisone-d5, were extracted from human plasma via liquid-liquid extraction using tert-Bu Me ether. The chromatog. separation was achieved on a Chromolith RP18e column using a mixture of acetonitrile and 2 mM ammonium formate (70:30, volume/volume) as the mobile phase at a flow rate of 0.7 mL/min. Quantitation was performed on a triple quadrupole mass spectrometer employing electrospray ionization technique, operating in multiple reaction monitoring and pos. ion mode. The precursors to product ion transitions monitored for fludrocortisone and IS were m/z 381.2 → 343.2 and 386.2 → 348.4, resp. The assay was validated with linear range of 40-3000 pg/mL. The intra- and inter-day precisions (relative standard deviation) were within 0.49-7.13 and 0.83-5.87%, resp. The proposed method was successfully applied to pharmacokinetic studies in humans. Copyright © 2015 John Wiley & Sons, Ltd. The experimental process involved the reaction of N,N-Dimethyl-3-phenyl-3-(pyridin-2-yl)propan-1-amine maleate(cas: 132-20-7).Application of 132-20-7
The Article related to fludrocortisone acetate plasma pharmacokinetics uhplc tandem mass spectrometry, uhplc-ms/ms, fludrocortisone, human plasma, method validation, pharmacokinetics, Pharmacology: Drug Metabolism and other aspects.Application of 132-20-7