《Zinc Photocages with Improved Photophysical Properties and Cell Permeability Imparted by Ternary Complex Formation》 was written by Basa, Prem N.; Barr, Chelsea A.; Oakley, Kady M.; Liang, Xiaomeng; Burdette, Shawn C.. Application of 1539-42-0This research focused onzinc photocage photophys cell permeability ternary complex; crystal structure. The article conveys some information:
Photocaged complexes can control the availability of metal ions to interrogate cellular signaling pathways. The authors describe a new photocage, {bis[(2-pyridyl)methyl]amino}(9-oxo-2-xanthenyl)acetic acid (XDPAdeCage, 1), which utilizes a 2-xanthone acetic acid group to mediate a photodecarboxylation reaction. XDPAdeCage photolyzes with a quantum yield of 27%, and binds Zn2+ with 4.6 pM affinity, which decreases by over 4 orders of magnitude after photolysis. For comparison to the previous approach to Zn2+ release via photodecarboxylation, the analogous photocage {bis[(2-pyridyl)methyl]amino}(m-nitrophenyl)acetic acid (DPAdeCage, 2), which uses a m-nitrobenzyl chromophore, was also prepared and characterized. The advantages of the 2-xanthone acetic acid chromophore include red-shifted excitation and a higher extinction coefficient at the preferred uncaging wavelength. The neutral ternary complex of [Zn(XDPAdeCage)]+ with the anionic ligand pyrithione is membrane permeable, which circumvents the need to utilize invasive techniques to introduce intracellular Zn2+ fluctuations. Using fluorescent imaging, the authors have confirmed transport of Zn2+ across membranes; in addition, RT-PCR experiments demonstrate changes in expression of Zn2+-responsive proteins after photolysis. The experimental process involved the reaction of Bis(pyridin-2-ylmethyl)amine(cas: 1539-42-0Application of 1539-42-0)
Bis(pyridin-2-ylmethyl)amine(cas: 1539-42-0) is a secondary amine with two picolyl substituents. The compound is a tridentate ligand in coordination chemistry and commonly used to produce Zn-based chemosensors/probes, such as Zinpry.Application of 1539-42-0