《Chemosensing of Guanosine Triphosphate Based on a Fluorescent Dinuclear Zn(II)-Dipicolylamine Complex in Water》 was published in Inorganic Chemistry in 2020. These research results belong to Bazany-Rodriguez, Ivan J.; Salomon-Flores, Maria K.; Bautista-Renedo, Joanatan M.; Gonzalez-Rivas, Nelly; Dorazco-Gonzalez, Alejandro. Recommanded Product: Bis(pyridin-2-ylmethyl)amine The article mentions the following:
Guanosine triphosphate (GTP) is a key biomarker of multiple cellular processes and human diseases. The new fluorescent dinuclear complex [Zn2(L)(S)][OTf]4, 1 (asym. ligand, L = 5,8-Bis{[bis(2-pyridylmethyl)amino] methyl}quinoline, S = solvent, and OTf = triflate anion) was synthesized and studied in-depth as a chemosensor for nucleoside polyphosphates and inorganic anions in pure water. Additions at neutral pH of nucleoside triphosphates, guanosine diphosphate, guanosine monophosphate, and pyrophosphate (PPi) to 1 quench its blue emission (λem = 410 nm) with a pronounced selectivity toward GTP over other anions, including ATP (ATP), uridine triphosphate (UTP), and cytidine triphosphate (CTP). The efficient quenching response by the addition of GTP was observed in the presence of coexisting species in blood plasma and urine with a detection limit of 9.2 μmol L-1. GTP also shows much tighter binding to the receptor 1 on a submicromolar level. On the basis of multiple spectroscopic tools (1H, 31P NMR, UV-vis, and fluorescence) and DFT calculations, the binding mode is proposed through three-point recognition involving the simultaneous coordination of the N7 atom of the guanosine motif and two phosphate groups to the two Zn(II) atoms. Spectroscopic studies, MS-ESI, and DFT suggested that GTP bound to 1 in 1:1 and 2:2 models with high overall binding constants of log β1 (1:1) = 6.05 ± 0.01 and log β2 = 10.91 ± 0.03, resp. The optical change and selectivity are attributed to the efficient binding of GTP to 1 by the combination of a strong electrostatic contribution and synergic effects of coordination bonds. Such GTP selectivity of an asym. metal-based receptor in water is still rare. The blue fluorescent Zn(II)-dinuclear complex, 1, based on the new 5,8-bis{[bis(2-pyridylmethyl)amino]methyl}quinoline ligand was designed, synthesized, and studied in depth as a chemosensor for nucleoside polyphosphates (NPPs) in neutral pure water. The fluorescence emission of 1 is affected strongly by the NPPs with pronounced selectivity toward guanosine triphosphate (GTP). This optical change is the result of the coordination of GTP to 1 through three-point recognition. The experimental part of the paper was very detailed, including the reaction process of Bis(pyridin-2-ylmethyl)amine(cas: 1539-42-0Recommanded Product: Bis(pyridin-2-ylmethyl)amine)
Bis(pyridin-2-ylmethyl)amine(cas: 1539-42-0) is a secondary amine with two picolyl substituents. As a tridentate ligand this compound provides three nitrogen donors that affords good selectivity for Zn2+ over biologically relevant metals such as Na+, K+, Mg2+ and Ca2+, and leaves coordination sites free for anion binding. Recommanded Product: Bis(pyridin-2-ylmethyl)amine