de Campos, Victoria M.;Andrade, Mariane A.;Maciel, Edvaldo V. S.;de Toffoli, Ana Lucia;Lancas, Fernando M. published 《Environmentally friendly analysis of sulphonamides in Brazilian honey through automated and miniaturised sample preparation coupled with LC-MS/MS》 in 2022. The article was appeared in 《Food Additives & Contaminants, Part A》. They have made some progress in their research.Formula: C10H9ClN4O2S The article mentions the following:
Increased use of environmentally friendly practices has become a trend in science because of the current awareness regarding climate change and related issues. Similarly for anal. chem., considering the development of greener methods for reducing the use of reagents and samples and also toxic waste generation. To meet such goals, automation, and miniaturization of sample preparation-a well-recognized laborious and time-consuming anal. step-are two promising strategies. This work associates the greener aspects of miniaturization and the performance of automated sample preparation Therefore, we proposed an anal. method using a miniaturized extraction column for pre-concentrating sulphamerazine, sulphamethazine, sulphamethoxazole, sulphadimethoxine, sulphathiazole, and sulphachlorpyridazine from honey and cleaning-up the samples. Several variables were optimized: extractive phase, loading flow, loading phase, and loading time. Under optimized conditions, the method showed adequate linearity between 5.0 and 60 ng g-1 with R > 0.99, and also good selectivity and recovery (114.6-124.1%) which are acceptable according to Brazilian legislation. Intra and inter-day precision were in the range 3.0-5.0%. Although sulfonamides were detected in one of the eight com. honey samples, the value was below the established MRL. The method showed efficiency, while also exhibiting greener characteristics resulting from miniaturization and automation, representing a promising environmentally friendly alternative for conventional sample preparation methods. To complete the study, the researchers used 4-Amino-N-(6-chloropyridazin-3-yl)benzenesulfonamide (cas: 80-32-0) .
4-Amino-N-(6-chloropyridazin-3-yl)benzenesulfonamide(cas: 80-32-0) can be introduced in the drinking water to fabricate aqueous liquid formulations for combating bacterial and protozoal in animals.Formula: C10H9ClN4O2S Besides, sulfachloropyridazine associated to tripelennamine hydrocholoride has been demonstrated to function as an anti-stress agent in poultry after vaccinations.