Paoletti, Fabiola;Sdogati, Stefano;Barola, Carolina;Giusepponi, Danilo;Moretti, Simone;Galarini, Roberta published 《Two-procedure approach for multiclass determination of 64 antibiotics in honey using liquid chromatography coupled to time-of-flight mass spectrometry》. The research results were published in《Food Control》 in 2022.COA of Formula: C10H9ClN4O2S The article conveys some information:
A “two-procedure approach” was developed to detect and confirm residues of 64 antibiotic substances in honey using liquid-chromatog. coupled to quadrupole time-of-flight mass spectrometry. Eight antimicrobial classes were determined (amphenicols, lincosamides, macrolides, nitroimidazoles, pleuromutilins, quinolones, sulfonamides and tetracyclines) together with single drugs. The procedures were validated in the range 0.2-50μg kg-1, obtaining recovery factors from 67 to 100% and coefficients of variation in within-lab reproducibility conditions lower than 20%. Limits of detection and quantification were in the ranges 0.1-3.3μg kg-1 and 0.2-11μg kg-1, resp., satisfying the current European levels for anal. control of antibiotic residues in honey. An extensive matrix effect study was performed considering acacia, chestnut and multiflower honey types. Method performances were confirmed through participation in proficiency tests obtaining z-scores between -0.7 and +1.08. The proposed approach was applied to honey samples collected in local markets and three out of fifty-five samples tested pos. The found mols. were sulfonamides (sulfamethazine and sulfathiazole) and tetracyclines (oxytetracycline and tetracycline) at concentrations lower than 2μg kg-1. Two out of three contaminated samples contained honey from extra-EU countries.4-Amino-N-(6-chloropyridazin-3-yl)benzenesulfonamide (cas: 80-32-0) were involved in the experimental procedure.
4-Amino-N-(6-chloropyridazin-3-yl)benzenesulfonamide(cas: 80-32-0) can be introduced in the drinking water to fabricate aqueous liquid formulations for combating bacterial and protozoal in animals.COA of Formula: C10H9ClN4O2S Besides, sulfachloropyridazine associated to tripelennamine hydrocholoride has been demonstrated to function as an anti-stress agent in poultry after vaccinations.