Varenina, Ivana;Bilandzic, Nina;Luburic, Djurdjica Bozic;Kolanovic, Bozica Solomun;Varga, Ines published 《High resolution mass spectrometry method for the determination of 13 antibiotic groups in bovine, swine, poultry and fish meat: An effective screening and confirmation analysis approach for routine laboratories》. The research results were published in《Food Control》 in 2022.Quality Control of 4-Amino-N-(6-chloropyridazin-3-yl)benzenesulfonamide The article conveys some information:
A screening and confirmatory method for the determination of 13 antibiotic groups including 91 substances was developed for muscle samples and validated using ultra-performance liquid chromatog. (UPLC) coupled to time-of-flight mass spectrometry. Optimization of the parameters was performed for every analyte and personal compound database library was created, comprising the mass spectra for following collision energies; 0, 10, 20 and 40 eV. Validation plan scheming and calculation of the parameters was performed using validation software, which included following performance characteristics; selectivity, trueness, precision, decision limit (CCα), detection capability (CCβ) and relative matrix effect. High resolution mass spectrometry operated in a narrow mass window and resolving power of 50,000 FWHM (2 GHz). Accurate mass measurements with mass accuracy below 10 ppm for all analytes were obtained in spiked muscle samples. The validation experiments showed good selectivity and specificity. The method was validated in the measurement range from 0.5 to 1000μg/kg, where a specific analyte mixed standard solution was prepared depending on the defined maximum residue limit (MRL) or lowest calibration level (LCL). For screening anal., detection capabilities (CCβ) below the set MRL values or at LCL concentration levels were defined. CCβ, as the concentration above which the sample can be considered suspect, ranged from 0.63 to 207.8μg/kg. For confirmation of the analyte, CCα was calculated at LCL or at the MRL, where it ranged from 0.38 to 14.3μg/kg for unauthorised substances and from 13.8 to 1492.6μg/kg for authorised substances. Relative within-laboratory reproducibility standard deviation ranged from 5.1 to 34.2%. Satisfactory z-score values in the range from -0.87 to 1.88 were obtained as a result of participating in four proficiency tests organized by European Union Reference Laboratories4-Amino-N-(6-chloropyridazin-3-yl)benzenesulfonamide (cas: 80-32-0) were involved in the experimental procedure.
4-Amino-N-(6-chloropyridazin-3-yl)benzenesulfonamide(cas: 80-32-0) can be introduced in the drinking water to fabricate aqueous liquid formulations for combating bacterial and protozoal in animals.Quality Control of 4-Amino-N-(6-chloropyridazin-3-yl)benzenesulfonamide Besides, sulfachloropyridazine associated to tripelennamine hydrocholoride has been demonstrated to function as an anti-stress agent in poultry after vaccinations.