On December 20, 2018, Fahrni, Christoph J.; McCallum, Adam M.; Morgan, Michael Thomas published a patent.Quality Control of Ethyl 6-cyanopicolinate The title of the patent was Preparation of substituted phenylthiazole compounds as zinc-selective fluorescent probes for emission-ratiometric imaging. And the patent contained the following:
The title compounds I [Z = II; X1-X4 = (independently) N and CH; Y1 = NR4, S, and O; Y2 = NR5, S, and O; R = Ar, OR4, N((CH2)qAr)2; R1-R4 = (independently) H, halo, alkyl, etc.; R5 = Cn-alkyl optionally substituted with OR4 or Ar; Ar = (independently) aryl and heteroaryl; n = 1-3; q = 1-3; and wherein any one of the aforementioned can be substituted or unsubstituted], useful for detecting zinc in a biol. sample (two-photo excitation microscopy (TPEM) or conventional fluorescence microscopy), were prepared E.g., a multi-step synthesis of III, starting from Et 6-bromopicolinate, was described. The latter was tested for Zn(II)-binding affinity and its analyte selectivity (data given). To evaluate the Zn(II)-dependent ratiometric response of III within the chem. complexity of a live cell, the authors performed a perfusion experiment with NIH 3T3 mouse fibroblasts as model system (data given). The experimental process involved the reaction of Ethyl 6-cyanopicolinate(cas: 97483-79-9).Quality Control of Ethyl 6-cyanopicolinate
The Article related to phenylthiazole preparation zinc selective fluorescent probe emission ratiometric imaging, two photo excitation microscopy tpem phenylthiazole preparation zinc probe, Heterocyclic Compounds (More Than One Hetero Atom): Thiazoles, Isothiazoles and other aspects.Quality Control of Ethyl 6-cyanopicolinate