Basic matrixes for the matrix-assisted laser desorption/ionization mass spectrometry of proteins and oligonucleotides was written by Fitzgerald, Michael C.;Parr, Gary R.;Smith, Lloyd M.. And the article was included in Analytical Chemistry in 1993.Category: pyridine-derivatives This article mentions the following:
In order to examine the importance of pH in the matrix-assisted laser desorption/ionization (MALDI) anal. of proteins and oligonucleotides, 37 highly substituted pyrimidine, pyridine, and benzene derivatives containing basic amino groups were screened as potential matrixes. Several of these compounds were useful for the preparation and anal. of samples under neutral and basic pH conditions. The matrix, 2-amino-4-methyl-5-nitropyridine, gave good results with small proteins (up to ∼12,000 Da). The best results with oligonucleotides were obtained with 2-amino-5-nitropyridine, which was a useful matrix for the anal. of mixed-base oligonucleotides smaller than 20 nucleotides in length and homopolymers of thymidine. In general, oligonucleotide anal. using basic matrixes was found to be subject to similar constraints of size and base composition that hold when acidic matrixes are used. A major advantage of these newly discovered matrix materials over the more commonly used acidic matrixes is that they extend the utility of MALDI to the anal. of acid-sensitive species. In the experiment, the researchers used many compounds, for example, 3-Amino-2,6-dimethoxypyridine (cas: 28020-37-3Category: pyridine-derivatives).
3-Amino-2,6-dimethoxypyridine (cas: 28020-37-3) belongs to pyridine derivatives. Pyridines are an important class of heterocycles and occur in polysubstituted forms in many naturally occurring biologically active compounds, drug molecules and chiral ligands. One of the examples of pyridines is the well-known alkaloid lithoprimidine, which is an A3 adenosine receptor antagonist and N,N-dimethylaminopyridine (DMAP) analog, commonly used in organic synthesis.Category: pyridine-derivatives