《Cu2+-based distance measurements by pulsed EPR provide distance constraints for DNA backbone conformations in solution》 was published in Nucleic Acids Research in 2020. These research results belong to Ghosh, Shreya; Lawless, Matthew J.; Brubaker, Hanna J.; Singewald, Kevin; Kurpiewski, Michael R.; Jen-Jacobson, Linda; Saxena, Sunil. Recommanded Product: 1539-42-0 The article mentions the following:
ESR (EPR) has become an important tool to probe conformational changes in nucleic acids. An array of EPR labels for nucleic acids are available, but they often come at the cost of long tethers, are dependent on the presence of a particular nucleotide or can be placed only at the termini. Site directed incorporation of Cu2+-chelated to a ligand, 2,2’dipicolylamine (DPA) is potentially an attractive strategy for site-specific, nucleotide independent Cu2+-labeling in DNA. To fully understand the potential of this label, we undertook a systematic and detailed anal. of the Cu2+-DPA motif using EPR and mol. dynamics (MD) simulations. We used continuous wave EPR experiments to characterize Cu2+ binding to DPA as well as optimize Cu2+ loading conditions. We performed double electron-electron resonance (DEER) experiments at two frequencies to elucidate orientational selectivity effects. Furthermore, comparison of DEER and MD simulated distance distributions reveal a remarkable agreement in the most probable distances. The results illustrate the efficacy of the Cu2+-DPA in reporting on DNA backbone conformations for sufficiently long base pair separations This labeling strategy can serve as an important tool for probing conformational changes in DNA upon interaction with other macromols. In the experiment, the researchers used Bis(pyridin-2-ylmethyl)amine(cas: 1539-42-0Recommanded Product: 1539-42-0)
Bis(pyridin-2-ylmethyl)amine(cas: 1539-42-0) is a secondary amine with two picolyl substituents. As a tridentate ligand this compound provides three nitrogen donors that affords good selectivity for Zn2+ over biologically relevant metals such as Na+, K+, Mg2+ and Ca2+, and leaves coordination sites free for anion binding. Recommanded Product: 1539-42-0