Gierten, Jakob published the artcileAutomated high-throughput heartbeat quantification in medaka and zebrafish embryos under physiological conditions, Related Products of pyridine-derivatives, the main research area is nifedipine cardioprotectant embryo heartbeat Oryzias Danio cardiovascular development.
Abstract: Accurate quantification of heartbeats in fish models is an important readout to study cardiovascular biol., disease states and pharmacol. However, dependence on anesthesia, laborious sample orientation or requirement for fluorescent reporters have hampered the use of high-throughput heartbeat anal. To overcome these limitations, we established an efficient screening assay employing automated label-free heart rate determination of randomly oriented, non-anesthetized medaka (Oryzias latipes) and zebrafish (Danio rerio) embryos in microtiter plates. Automatically acquired bright-field data feeds into an easy-to-use HeartBeat software with graphical user interface for automated quantification of heart rate and rhythm. Sensitivity of the assay was demonstrated by profiling heart rates during entire embryonic development. Our anal. revealed rapid adaptation of heart rates to temperature changes, which has implications for standardization of exptl. layout. The assay allows scoring of multiple embryos per well enabling a throughput of >500 embryos per 96-well plate. In a proof of principle screen for compound testing, we captured concentration-dependent effects of nifedipine and terfenadine over time. Our novel assay permits large-scale applications ranging from phenotypic screening, interrogation of gene functions to cardiovascular drug development.
Scientific Reports published new progress about Anesthesia. 21829-25-4 belongs to class pyridine-derivatives, name is Dimethyl 2,6-dimethyl-4-(2-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate, and the molecular formula is C17H18N2O6, Related Products of pyridine-derivatives.