Guo, Leilei;Yang, Wenlong;Cheng, Xi;Fan, Zhixia;Chen, Ximeng;Ge, Feng;Dai, Yijun published 《Degradation of neonicotinoid insecticide acetamiprid by two different nitrile hydratases of Pseudaminobacter salicylatoxidans CGMCC 1.17248》. The research results were published in《International Biodeterioration & Biodegradation》 in 2021.Quality Control of 3-Cyanopyridine The article conveys some information:
Acetamiprid is a neonicotinoid insecticide used worldwide that has caused environmental pollution and adverse effects on ecosystems. Here, a novel bacterium, Pseudaminobacter salicylatoxidans CGMCC 1.17248, was isolated to rapidly degrade acetamiprid to IM-1-2 via hydration. Gene cloning and overexpression demonstrated that two nitrile hydratases, AnhA and AnhB, converted acetamiprid to IM-1-2. Escherichia coli overexpressing AnhA and AnhB degraded 98.1% and 94.0% of acetamiprid (1.0 mmol L-1) in 5 min and 8 h, resp. The pure AnhA and AnhB had a Vmax value of 14.12 and 1.20 μmol mg-1 min-1, resp., and a Km value of 1.02 mmol L-1 and 2.95 mmol L-1, resp. Compared with AnhA, AnhB had broad pH stability, as well as metal ions and organic solvents tolerance. Expression of AnhA and AnhB was induced by decreasing the nutrient concentration of culture broth and addition of urea and therefore significantly enhanced acetamiprid degradation of CGMCC 1.17248. qPCR indicated that the expression of AnhA and AnhB under the cultured conditions of 1/15 lysogeny broth or 0.5% urea addition was improved by from 2.2 to 5.3 times. The results presented herein will facilitate development of bioremediation agents for acetamiprid pollution and understanding of the functional role of bacterial nitrile hydratase.3-Cyanopyridine (cas: 100-54-9) were involved in the experimental procedure.
3-Cyanopyridine(cas: 100-54-9) is an antimicrobial agent that can be used in the treatment of infectious diseases.Quality Control of 3-Cyanopyridine It has been shown to be effective against a variety of bacteria, including methicillin-resistant Staphylococcus aureus (MRSA) and Streptococcus pneumoniae.