Hayes, Heather B. published the artcileNovel method for action potential measurements from intact cardiac monolayers with multiwell microelectrode array technology, COA of Formula: C17H18N2O6, the main research area is cardiac monolayer action potential multiwell microelectrode array.
The cardiac action potential (AP) is vital for understanding healthy and diseased cardiac biol. and drug safety testing. However, techniques for high throughput cardiac AP measurements have been limited. Here, we introduce a novel technique for reliably increasing the coupling of cardiomyocyte syncytium to planar multiwell microelectrode arrays, resulting in a stable, label-free local extracellular action potential (LEAP). We characterized the reliability and stability of LEAP, its relationship to the field potential, and its efficacy for quantifying AP morphol. of human induced pluripotent stem cell derived and primary rodent cardiomyocytes. Rise time, action potential duration, beat period, and triangulation were used to quantify compound responses and AP morphol. changes induced by genetic modification. LEAP is the first high throughput, non-invasive, label-free, stable method to capture AP morphol. from an intact cardiomyocyte syncytium. LEAP can accelerate our understanding of stem cell models, while improving the automation and accuracy of drug testing.
Scientific Reports published new progress about Action potential. 21829-25-4 belongs to class pyridine-derivatives, name is Dimethyl 2,6-dimethyl-4-(2-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate, and the molecular formula is C17H18N2O6, COA of Formula: C17H18N2O6.