He, Chengzhi; Li, Shuai; Gao, Xiaoqing; Xiao, Adam; Hu, Chunguang; Hu, Xiaodong; Hu, Xiaotang; Li, Hongbin published the artcile< Direct observation of the fast and robust folding of a slipknotted protein by optical tweezers>, Computed Properties of 2127-03-9, the main research area is slipknotted protein folding optical tweezer direct observation.
Understanding the folding mechanism of knotted and slipknotted proteins has attracted considerable interest. Due to their topol. complexity, knotted and slipknotted proteins are predicted to fold slowly and involve large topol. barriers. Mol. dynamics simulation studies suggest that a slipknotted conformation can serve as an important intermediate to help greatly reduce the topol. difficulty during the folding of some knotted proteins. Here we use a single mol. optical tweezers technique to directly probe the folding of a small slipknotted protein AFV3-109. We found that stretching AFV3-109 can lead to the untying of the slipknot and complete unfolding of AFV3-109. Upon relaxation, AFV3-109 can readily refold back to its native slipknot conformation with high fidelity when the stretching force is lower than 6 pN. The refolding of AFV3-109 occurs in a sharp two-state like transition. Our results indicate that, different from knotted proteins, the folding of a slipknotted protein like AFV3-109 can be fast, and may not necessarily involve a high topol. barrier.
Nanoscale published new progress about Denaturation. 2127-03-9 belongs to class pyridine-derivatives, and the molecular formula is C10H8N2S2, Computed Properties of 2127-03-9.