Operationally Simple Regioselective 5′-Phosphorylation of Unprotected 5-Ethynyl-2′-deoxyuridine Analogues was written by Hilko, David H.;Bornaghi, Laurent F.;Poulsen, Sally-Ann. And the article was included in Australian Journal of Chemistry in 2020.Reference of 628-13-7 This article mentions the following:
Here, we present the development of a straightforward methodol. to regioselectively phosphorylate the 5′-OH group of unprotected nucleosides. We employ cyclosaligenyl phosphoramidite reagents together with pyridinium trifluoroacetate as activator, followed by in-situ oxidation to prepare a panel of novel nucleoside-based chem. probes, Pro-Label compounds Alternative procedures for this transformation are available, but are limited in number and scope. Furthermore, the benefits of the new methodol. include milder reaction conditions, wider solvent applicability, and, by avoiding sensitive reagents, a more straightforward handling of reagents, reactions, and workup processes. The panel of novel cyclosaligenyl phosphotriester uridine Pro-Labels have variable 2′ substituents (H, F, Cl, Br, I) as well as four different cyclosaligenyl groups that would span a range of half-lives for in-vitro applications. In the experiment, the researchers used many compounds, for example, Pyridinehydrochloride (cas: 628-13-7Reference of 628-13-7).
Pyridinehydrochloride (cas: 628-13-7) belongs to pyridine derivatives. The ring atoms in the pyridine molecule are sp2-hybridized. The nitrogen is involved in the π-bonding aromatic system using its unhybridized p orbital. The lone pair is in an sp2 orbital, projecting outward from the ring in the same plane as the σ bonds. Reduced pyridines, namely tetrahydropyridines, dihydropyridines and piperidines, are found in numerous natural and synthetic compounds. The synthesis and reactivity of these compounds have often been driven by the fact many of these compounds have interesting and unique pharmacological properties. Reference of 628-13-7