TCF-imidazo[1,5-a]pyridine: A potential robust ratiometric fluorescent probe for glutathione detection with high selectivity was written by Hou, Peng;Sun, Jingwen;Wang, Haijun;Liu, Lei;Zou, Liwei;Chen, Song. And the article was included in Sensors and Actuators, B: Chemical in 2020.Formula: C12H9NO This article mentions the following:
A novel fluorescent probe, TCF-IPY, has been designed and developed for highly selective and ratiometric detection of GSH based on the extend of the p-conjugation system of imidazo[1,5-a]pyridine IPY-CHO. Upon addition of GSH, TCF-IPY displayed remarkable fluorescence variations (approx. 949-fold ratio changes) from 603 nm to 475 nm, thereby enabling GSH detection in a fine ratiometric manner. Moreover, TCF-IPY exhibited excellent selectivity toward GSH over other biol. related species including Cys and Hcy. The huge blue shifts both in absorption (259 nm) and emission spectra (128 nm), low cytotoxicity, high sensitivity (97 nM) and rapid response (240 s) of TCF-IPY make it a robust mol. tool for endogenous GSH investigation. Furthermore, TCF-IPY was also successfully used for ratiometric imaging of endogenous GSH in living MCF-7 cells and zebrafish. In the experiment, the researchers used many compounds, for example, Phenyl(pyridin-2-yl)methanone (cas: 91-02-1Formula: C12H9NO).
Phenyl(pyridin-2-yl)methanone (cas: 91-02-1) belongs to pyridine derivatives. Pyridine has a conjugated system of six π electrons that are delocalized over the ring. The molecule is planar and, thus, follows the Hückel criteria for aromatic systems. One of the examples of pyridines is the well-known alkaloid lithoprimidine, which is an A3 adenosine receptor antagonist and N,N-dimethylaminopyridine (DMAP) analog, commonly used in organic synthesis.Formula: C12H9NO