In 2022,Ibrahim, Adel Ehab; El Deeb, Sami; Abdellatef, Hisham Ezzat; Hendawy, Hassan A. M.; El-Abassy, Omar M.; Ibrahim, Hany published an article in Separations. The title of the article was ãEco-Friendly and Sensitive HPLC and TLC Methods Validated for the Determination of Betahistine in the Presence of Its Process-Related Impurityã?Name: 2-(2-Hydroxyethyl)pyridine The author mentioned the following in the article:
Reducing the amounts consumed of organic solvents while keeping good chromatog. performance has been a significant step towards the greening of anal. methodologies. When sodium dodecyl sulfate (SDS) and Brij-35 surfactants are combined in a mobile phase, they can be used as a green alternative to organic modifiers. Surfactants have numerous advantages, including low cost and toxicity, safe environmental disposal, and unique selectivity, in addition to high solubilization capabilities. In this research, two highly selective chromatog. methods were adopted for the determination of betahistine (BHS) in the presence of its pharmacopeial impurity 2-(2-hydroxyethyl)pyridine (HEP). A solvent-free HPLC method was validated, in which the mixture was separated using a C18 column (3.5μm, 75.0 x 4.6 mm) and a mobile phase composed of 0.01 M Brij-35, 0.12 M SDS, and 0.02 M disodium hydrogen phosphate adjusted to a pH of 5.5 using phosphoric acid. The flow rate was 1.5 mL min-1 and the resolved peaks were detected at 260 nm. Another HPTLC-densitometric method was validated using HPTLC aluminum plates coated with silica gel 60 F254 as the stationary phase and a developing system consisting of methylene chloride/methanol/ethyl acetate/ammonia (at a ratio of 5:2:2:0.2 by volume); the separated bands were scanned at 260 nm.2-(2-Hydroxyethyl)pyridine(cas: 103-74-2Name: 2-(2-Hydroxyethyl)pyridine) was used in this study.
2-(2-Hydroxyethyl)pyridine(cas: 103-74-2) belongs to pyridine. Pyridine is very deactivated towards electrophilic substitution with respect to benzene. For this reason classical formylation, using methods such as the Gattermann or Vilsmeier reactions, are not generally successful. Name: 2-(2-Hydroxyethyl)pyridine