Structural and biochemical characterisation of the Providencia stuartii arginine decarboxylase shows distinct polymerisation and regulation was written by Jessop, Matthew;Huard, Karine;Desfosses, Ambroise;Tetreau, Guillaume;Carriel, Diego;Bacia-Verloop, Maria;Mas, Caroline;Mas, Philippe;Fraudeau, Angelique;Colletier, Jacques-Philippe;Gutsche, Irina. And the article was included in Communications Biology in 2022.Synthetic Route of C8H10NO6P The following contents are mentioned in the article:
Bacterial homologous lysine and arginine decarboxylases play major roles in the acid stress response, physiol., antibiotic resistance and virulence. The Escherichia coli enzymes are considered as their archetypes. Whereas acid stress triggers polymerization of the E. coli lysine decarboxylase LdcI, such behavior has not been observed for the arginine decarboxylase Adc. Here we show that the Adc from a multidrug-resistant human pathogen Providencia stuartii massively polymerises into filaments whose cryo-EM structure reveals pronounced differences between Adc and LdcI assembly mechanisms. While the structural determinants of Adc polymerization are conserved only in certain Providencia and Burkholderia species, acid stress-induced polymerization of LdcI appears general for enterobacteria. Anal. of the expression, activity and oligomerisation of the P. stuartii Adc further highlights the distinct properties of this unusual protein and lays a platform for future investigation of the role of supramol. assembly in the superfamily or arginine and lysine decarboxylases. This study involved multiple reactions and reactants, such as (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7Synthetic Route of C8H10NO6P).
(4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7) belongs to pyridine derivatives. Pyridines are an important class of heterocycles and occur in polysubstituted forms in many naturally occurring biologically active compounds, drug molecules and chiral ligands. Many analogues of pyridine are known where N is replaced by other heteroatoms . Substitution of one C鈥揌 in pyridine with a second N gives rise to the diazine heterocycles (C4H4N2), with the names pyridazine, pyrimidine, and pyrazine.Synthetic Route of C8H10NO6P