Kelani, Khadiga M.; Hegazy, Maha A.; Hassan, Amal M.; Tantawy, Mahmoud A. published an article in 2021, the title of the article was Determination of naphazoline HCl, pheniramine maleate and their official impurities in eye drops and biological fluid rabbit aqueous humor by a validated LC-DAD method.COA of Formula: C20H24N2O4 And the article contains the following content:
A simple RP-HPLC-DAD method was developed and validated, as per the ICH guidelines, for simultaneous determination of naphazoline HCl (NPZ) & pheniramine maleate (PHN) along with three of their official impurities. Chromatog. separation was performed on a hypersil ODS column (5 mm, 250-4.6 mm i.d.) with isocratic elution using phosphate buffer pH 6.0: acetonitrile (70 : 30, volume/volume) as mobile phase, at a flow rate of 1.0 mL min-1 and UV detection at 260.0 nm. The developed method was found to be linear over the concentration ranges of 5.00-45.00 Μg mL-1 for NPZ and NPZ impurity B and 10.00-110.00 Μg mL-1, 10-70 Μg mL-1 and 10-120 Μg mL-1 for PHN, and PHN impurity A and B, resp., with correlation coefficient values <0.999 for the five cited compounds The method was confirmed to be accurate, robust and precise with RSD >2.0%. LOD and LOQ values for the five cited compounds were calculated Moreover, the method was also validated in rabbit aqueous humor as per the US food and drug administration (FDA) bioanal. validation guidelines. Finally, the proposed method was applied for the anal. of the two drugs along with their impurities in dosage form and spiked aqueous humor samples. The experimental process involved the reaction of N,N-Dimethyl-3-phenyl-3-(pyridin-2-yl)propan-1-amine maleate(cas: 132-20-7).COA of Formula: C20H24N2O4
The Article related to naphazoline hcl pheniramine maleate eye drop biol fluid, Biochemical Methods: Immunological and other aspects.COA of Formula: C20H24N2O4