Li, Ze; Li, Wenwei; Lu, Maolin; Bess, Julian Jr; Chao, Cara W.; Gorman, Jason; Terry, Daniel S.; Zhang, Baoshan; Zhou, Tongqing; Blanchard, Scott C.; Kwong, Peter D.; Lifson, Jeffrey D.; Mothes, Walther; Liu, Jun published the artcile< Subnanometer structures of HIV-1 envelope trimers on aldrithiol-2-inactivated virus particles>, Recommanded Product: 1,2-Di(pyridin-2-yl)disulfane, the main research area is .
Abstract: The HIV-1 envelope glycoprotein (Env) trimer, composed of gp120 and gp41 subunits, mediates viral entry into cells. Recombinant Env trimers have been studied structurally, but characterization of Env embedded in intact virus membranes has been limited to low resolution Here, we deploy cryo-electron tomog. and subtomogram averaging to determine the structures of Env trimers on aldrithiol-2 (AT-2)-inactivated virions in ligand-free, antibody-bound and CD4-bound forms at subnanometer resolution Tomog. reconstructions document mol. features consistent with high-resolution structures of engineered soluble and detergent-solubilized Env trimers. One of three conformational states previously predicted by smFRET was not observed by cryo-ET, potentially owing to AT-2 inactivation. We did observe Env trimers to open in situ in response to CD4 binding, with an outward movement of gp120-variable loops and an extension of a critical gp41 helix. Overall features of Env trimer embedded in AT-2-treated virions appear well-represented by current engineered trimers.
Nature Structural & Molecular Biology published new progress about 2127-03-9. 2127-03-9 belongs to class pyridine-derivatives, and the molecular formula is C10H8N2S2, Recommanded Product: 1,2-Di(pyridin-2-yl)disulfane.