Lim, Xin Rui published the artcileFast voltage-dependent sodium (Nav) currents are functionally expressed in mouse corpus cavernosum smooth muscle cells, Synthetic Route of 21829-25-4, the publication is British Journal of Pharmacology (2022), 179(5), 1082-1101, database is CAplus and MEDLINE.
Corpus cavernosum smooth muscle (CCSM) exhibits phasic contractions that are coordinated by ion channels. Mouse models are commonly used to study erectile dysfunction, but there are few published electrophysiol. studies of mouse CCSM. We describe the voltage-dependent sodium (NaV) currents in mouse CCSM and investigate their function. We used electrophysiol., pharmacol. and immunocytochem. methods to study the NaV currents in isolated CCSM cells from C57BL/6 mice. Tension measurements were carried out using crural sections of the corpus cavernosum in whole tissue. Fast, voltage-dependent, sodium currents in mouse CCSM were induced by depolarising steps. Steady-state activation and inactivation curves revealed a window current between -60 and -30 mV. Two populations of NaV currents, â²TTX-sensitiveâ?and â²TTX-insensitiveâ? were identified. TTX-sensitive currents showed 48% block with the NaV channel subtype-specific blockers ICA-121431 (NaV1.1-1.3), PF-05089771 (NaV1.7) and 4,9-anhydro-TTX (NaV1.6). TTX-insensitive currents were resistant to blockade by A803467, specific for NaV1.8 channels. Immunocytochem. confirmed expression of NaV1.5 and NaV1.4 in freshly dispersed CCSM cells. Veratridine, a NaV channel activator, reduced time-dependent inactivation of NaV currents and increased duration of evoked action potentials. Veratridine induced phasic contractions in CCSM strips, reversible with TTX and nifedipine but not KB-R7943. There are fast, voltage-dependent, sodium currents in mouse CCSM. Stimulation of these currents increased contractility of CCSM in vitro, suggesting an involvement in detumescence and potentially providing a clin. relevant target in erectile dysfunction. Further work will be necessary to define its role.
British Journal of Pharmacology published new progress about 21829-25-4. 21829-25-4 belongs to pyridine-derivatives, auxiliary class Membrane Transporter/Ion Channel,Calcium Channel, name is Dimethyl 2,6-dimethyl-4-(2-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate, and the molecular formula is C17H18N2O6, Synthetic Route of 21829-25-4.
Referemce:
https://en.wikipedia.org/wiki/Pyridine,
Pyridine | C5H5N – PubChem