Louw, Stefan published the artcileSerial coupling of reversed-phase and hydrophilic interaction liquid chromatography to broaden the elution window for the analysis of pharmaceutical compounds, Application In Synthesis of 18437-58-6, the publication is Journal of Chromatography A (2008), 1208(1-2), 90-94, database is CAplus and MEDLINE.
It is presently a common practice in drug discovery to analyze samples by reversed-phase liquid chromatog. (RPLC) and hydrophilic interaction chromatog. (HILIC). To increase throughput, HILIC was connected in series to RPLC by a T-piece with make-up flow. The first column is a 2 mm I.D. column having an optimal flow between 0.1 and 0.2 mL/min. Via the T-piece, the flow for the second dimension column with an I.D. of 4.6 mm is adjusted to 1.5-2.0 mL/min with a high acetonitrile content (i.e. â?0%) mobile phase. Therefore, even in gradient RPLC anal. starting with a mobile phase with high water content, the HILIC column is always operated at high acetonitrile concentration which is required to obtain retention on the HILIC column. The performance of the hyphenated RPLC/HILIC set-up is illustrated with the anal. of 2 model samples of pharmaceutical interest. Optimization of the conditions in the HILIC dimension is discussed.
Journal of Chromatography A published new progress about 18437-58-6. 18437-58-6 belongs to pyridine-derivatives, auxiliary class Pyridine,Amine, name is 4-Amino-2-picoline, and the molecular formula is C6H8N2, Application In Synthesis of 18437-58-6.
Referemce:
https://en.wikipedia.org/wiki/Pyridine,
Pyridine | C5H5N – PubChem