Mishra, Abhishek Kumar published the artcileFunctional insights into voltage gated proton channel (Hv1) in bull spermatozoa, Recommanded Product: Dimethyl 2,6-dimethyl-4-(2-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate, the main research area is cAMP PKA voltage gated proton channel biomarker spermatozoa; Capacitation, 2-GBI, zinc, AEA, bull, spermatozoa; Catsper; Hv1; PKA; Sperm motility; sAC.
Acid extrusion and intracellular alkalisation are the key events during sperm capacitation and these are mediated through proton gated channels (Hv1). Role of Hv1 in regulating sperm motility, capacitation and acrosome reaction has been documented in human spermatozoa; but no such data is available in bull spermatozoa; therefore, the present study was undertaken in Hariana bull spermatozoa. Sixty four ejaculates were collected from four Hariana bulls to investigate the functional involvement of Hv1 in regulation of sperm motility, capacitation and acrosome reaction in bull spermatozoa. Immunoblotting revealed the presence of a single band of 31.8 kDa corresponding to Hv1 in Hariana bull spermatozoa and immunofluorescence confirmed the pos. immune-reactivity at principal piece of spermatozoa for Hv1. Functional study was carried out using 200μM 2- Guadinobenzimidazole (2-GBI,selective Hv1 blocker) and 1 mM zinc chloride (potent Hv1 blocker), and 0.3μM Anandamide (AEA), an activator of Hv1. Blocking as well as activation of Hv1 resulted in significant (P < 0.05) reduction in sperm livability, spermatozoa having intact membrane, intact acrosome, and high mitochondrial transmembrane potential (MTP). Further studies are required to find out the possible relationship between Hv1 channels and other channels in regulating spermatozoa functions. Theriogenology published new progress about Acrosome. 21829-25-4 belongs to class pyridine-derivatives, name is Dimethyl 2,6-dimethyl-4-(2-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate, and the molecular formula is C17H18N2O6, Recommanded Product: Dimethyl 2,6-dimethyl-4-(2-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate.