Renaud, Justin B. published the artcileNormalization of LC-MS mycotoxin determination using the N-alkylpyridinium-3-sulfonates (NAPS) retention index system, Category: pyridine-derivatives, the publication is Journal of Chromatography A (2021), 461901, database is CAplus and MEDLINE.
A major challenge for LC-MS anal. is the ability to compare data between laboratories and across instrument platforms. Currently, mycotoxin determination relies on dereplication strategies based on physicochem. properties such as the m/z of the precursor and product ions. Unlike these intrinsic properties, retention time (tR) is an extrinsic property impacted by LC conditions, including mobile phases and column chem., making exchange of data between groups difficult. To address this, we are introducing the concept of incorporating an electrospray compatible, retention index (RI) system based on a series of N-alkylpyridinium-3-sulfonates (NAPS) into routine mycotoxin determination These standards of differing alkyl chain length span RI units from 100 to 2000, are UV active and have fixed pos. and neg. charges for electrospray ionization in either mode. Using high resolution LC-MS/MS, the RIs of 96 mycotoxins and fungal secondary metabolites were normalized as a proof of concept with the NAPS RI system under multiple pH, column and gradient chromatog. conditions. This method was then applied to the anal. of a crude extract of Penicillium roqueforti, where we were able to decrease the number of false positives by implementing an RI filter as well as a secondary correction factor. Addnl., we developed software that allows users to convert published RIs to a predicted tR values. Integration of the NAPS RI system into routine LC-MS anal. will improve compound identifications and help facilitate ease of data sharing.
Journal of Chromatography A published new progress about 636-73-7. 636-73-7 belongs to pyridine-derivatives, auxiliary class Pyridine,Sulfonic acid, name is Pyridine-3-sulfonic acid, and the molecular formula is C5H5NO3S, Category: pyridine-derivatives.
Referemce:
https://en.wikipedia.org/wiki/Pyridine,
Pyridine | C5H5N – PubChem